Functional Analytics Core

项目总结 近年来，我们通过CRISPR/Cas9诱导的操纵单个细胞遗传密码的能力 突变和高通量数据的生成已经迅速推进。然而，主要瓶颈是 CRISPR/Cas9的使用在技术上仍然具有挑战性，特别是在角质形成细胞中，角质形成细胞 与其他类型的细胞相比，更具挑战性的是转基因。此外，大多数研究小组的能力 分析高通量数据，并将其整合到有意义的生物和病理背景中 有限的。因此，对CRISPR/CAS9服务的专门知识和能力都有巨大的需求 以及生物信息资源来翻译大量信息，并将其与相关的生物或 特定于疾病的情况。为了填补这一关键空白，密歇根大学皮肤生物学和资源部 中心(UM-SBDRC)功能分析核心(FAC)将提供复杂的基础设施、资源、 在密歇根大学UM-SBDRC下的专业知识和培训机会。联邦航空局正在排队 NIAMS的使命是提供所需的研究基础设施、汇集的设施、服务和资源，以 进行皮肤生物学和疾病研究的研究人员小组。外交事务委员会的目标是 加速、增强和丰富正在进行的基础研究和翻译研究的有效性，并帮助 将外部研究人员带入皮肤生物学领域。这将通过以下两个目标来实现 FAC：在目标1中，我们在角质形成细胞中提供CRISPR/Cas9基因组编辑服务以生成 敲除和敲入角质形成细胞以及突变角质形成细胞系的功能特征， 和目标2，在那里我们提供系统生物学分析、疾病和细胞因子特异性基因 表情分析和训练。这些服务并不广泛地用于皮肤研究 社区，以及通过此核心提供的服务、资源和技术将提供UM- SBDRC成员具有高度创新的方法，将促进和加快正在进行的研究和 帮助吸引新的研究团队进入皮肤研究领域。

PROJECT SUMMARY In recent years our abilities to manipulate the genetic code of individual cells, through CRISPR/Cas9 induced mutagenesis, and generation of high-throughput data have rapidly advanced. However, major bottlenecks remain; the use of CRISPR/Cas9 remains technically challenging, particularly in keratinocytes, which are much more challenging to transfect than other cell-types. Furthermore, the ability of most research groups to analyze high-throughput data and incorporate it into a meaningful biologic and pathologic context remains limited. Therefore, there is an enormous need for expertise and capabilities both for CRISPR/Cas9 services as well as bioinformatic resources to translate the vast information and connect it with relevant biological or disease-specific situation. To fill this critical gap, the University of Michigan Skin Biology and Resource-based Center (UM-SBDRC) Functional Analytics Core (FAC) will provide sophisticated infrastructure, resources, expertise, and training opportunities at the University of Michigan under the UM-SBDRC. The FAC is in line with the NIAMS mission to provide needed research infrastructure, pooled facilities, services, and resources to groups of investigators conducting research on skin biology and diseases. The goal of the FAC is to accelerate, enhance, and enrich the effectiveness of ongoing basic and translational research, and to help bring outside investigators into the field of cutaneous biology. This will be achieved through the two aims of the FAC: In Aim 1 where we provide CRISPR/Cas9 genome editing services in keratinocytes for generation of both knock-out and knock-in keratinocytes as well as functional characterization of mutant keratinocyte lines, and Aim 2, where we provide access to systems biology analyses, disease- and cytokine-specific gene expression profiling, and training. These services are not widely available for the cutaneous research community, and the services, resources, and techniques made available through this Core will provide UM- SBDRC Members with highly innovative approaches that will promote and accelerate ongoing research and help attract new research teams into the fold of cutaneous research.