Paper-based high shear hemostatic analytical device

项目总结/摘要 有许多疾病（例如心律失常、冠状动脉疾病和感染）和情况（例如， 医疗器械、创伤和手术），需要快速、准确的即时止血测试， 特别是在调节抗血栓药物或输血后。然而， 在资源有限的环境中，如低收入和中等收入国家（LMIC）、农村诊所、战斗 环境，以及许多其他。这是因为目前的止血测定需要电力， 资本支出、熟练的技术人员、大型设备，并且可能很慢。如心血管疾病 随着低收入国家的增加，迫切需要为这些资源较低的国家提供创新工具， 环境.作为替代方案，基于微流体纸的分析装置（Microfluidic-paper based analytical devices，简称PAD）已经被广泛应用。 开发用于低资源设置作为护理点技术，因为这些是快速的，具有成本效益的， 便携式、一次性、易于使用，并且通常需要电力。这些装置包括快速抗原 为COVID-19开发的测试，为教育健康提供关键的快速反馈， 决策这些几乎可以用在任何地方，相对较少的技能。我们的目标是开发一个类似的 我们称之为纸基凝血分析测试（P-CAT）。目前的TMPAD是 止血分析有限，因为它们通常会产生低流量条件，无法测试许多 主要止血过程/途径。我们开发了一种快速流动的垫片， 整合到P-CAT中，使我们能够研究参与P-CAT的途径谱。 仅用一滴（微升）血液止血。P-CAT可以以非常低的成本快速创建， 从而产生高吞吐量数据。在这里，我们将开发P-CAT来测试血液样本， 对1）主要（血小板依赖性）止血途径和2） 二次止血中的内源性和外源性凝血级联。如果成功，我们将扩大 在P-CAT上，将额外的生物标志物纳入自动化护理点设备中， 对实现便携、快速和廉价的止血测试具有重大影响， 分布广泛。

PROJECT SUMMARY/ABSTRACT There are many diseases (e.g. arrhythmias, coronary artery disease, and infections) and situations (e.g. medical devices, trauma, and surgery) that require rapid and accurate point-of-care hemostatic tests, especially when regulating antithrombotics or after a blood transfusion. However, there are few options in resource-limited environments like low- and middle-income countries (LMICs), rural clinics, combat environments, and many others. This is because current hemostatic assays require electrical power, capital expense, skilled technicians, large equipment, and can be slow. As cardiovascular disease increases in LMICs, there is an urgent need to provide innovative tools for these lower resource environments. As an alternative, microfluidic-paper based analytical devices (PAD)s have been developed for low resource settings as a point-of-care technology since these are rapid, cost-effective, portable, disposable, eas-to-use, and typically require electricity. Such devices include the rapid antigen tests developed for COVID-19, which provide critical rapid feedback for making educated health decisions. These can be used almost anywhere with relatively little skill. Our goal is to develop a similar point-of-care tool that we call the Paper-based Clotting Analysis Test (P-CAT). Current PADs are limited for hemostatic analysis since they typically produce low flow conditions that can’t test many of the processes/pathways in primary hemostasis. We have developed a fast-flow PAD that will be incorporated into the P-CAT, enabling us to investigate the spectrum of pathways involved in hemostasis with only a drop (microliters) of blood. P-CATs can be created rapidly and at very little cost, thereby producing high throughput data. Here, we will develop the P-CAT to test blood samples with high specificity and and sensitivity to 1) pathways in primary (platelet-dependent) hemostasis, and 2) intrinsic and extrinsic coagulation cascades in secondary hemostasis. If successful, we will expand upon the P-CAT to incorporate additional biomarkers in an automated point-of-care device that could have a major impact on enabling portable, rapid, and inexpensive tests for hemostasis that can be widely distributed.