Metabolomics Core

项目摘要/摘要--核心B 异基因造血干细胞移植(allo-hct)可以提供一种治疗慢性感染的方法， 疾病，或血癌。然而，它的治疗作用受到相关毒性的限制。 由移植物抗宿主病(GVHD)引起。移植物抗宿主病最突出的病症包括 胃肠道受损。我们小组的最新结果显示， GVHD受肠道微生物组的性质和代谢特性的影响。此外，我们发现， 促进促进淀粉代谢为丁酸盐的微生物群落和重新编程 BMT后宿主的胆汁酸代谢可改善甚至预防GVDH。该措施和 新陈代谢的量化对于解决相关的实验目标至关重要。 研究建议。代谢组学核心的目的是支持以下研究项目 在这些研究的基础上，检测和量化小鼠粪便中这些途径的代谢物，细菌 相关临床患者的人群、人类上皮细胞和纵向粪便样本 审判。此外，该核心还将提供对宿主和微生物组代谢的全局洞察，使用STRATE 同位素示踪技术和非靶向代谢组全谱分析。通过集中开发， 代谢组学方法的维护和实施，这一核心将提供最先进的 促进数据收集和分析的能力，同时确保项目之间的一致性。

PROJECT SUMMARY/ABSTRACT – CORE B Allogenic hematopoietic stem cell transplant (allo-HCT) can provide a cure for chronic infections, genetic diseases, or cancers of the blood. However, its therapeutic utility is limited by associated toxicities resulting from graft versus host disease (GVHD). Among the most prominent morbidities of GVHD is damage to the gastrointestinal tract. Recent results from our groups have revealed that the severity of GVHD is impacted by the nature and metabolic properties of the gut microbiome. Further, we found that promotion of microbial communities that facilitate the metabolism of starch into butyrate and reprogram bile acid metabolism of the host ameliorate or even prevent GVDH following BMT. The measure and quantitation of metabolism is essential to address the associated experimental aims across the research proposals. The purpose of the Metabolomics core is to support the research projects that build on these studies to detect and quantify metabolites from these pathways in stool from mice, bacterial populations, human epithelial cells, and longitudinal stool samples of patients in the associated clinical trial. Further, this core will also provide global insights into host and microbiome metabolism using stable isotope tracing technologies and untargeted metabolome-wide profiling. By centralizing the development, maintenance, and implementation of metabolomics methods, this core will provide state-of-the-art capabilities to facilitate data collection and analysis while ensuring consistency across projects.