Implementation and Evaluation of Two SARS-CoV-2 Assays for Companion Animal Testing to Expand Vet-LIRN COVID-19 Capacity

项目摘要 严重急性呼吸综合征冠状病毒2（SARS-CoV-2）是一种人畜共患疾病， 除了在人类大流行中的重要性外，还造成了溢出感染， 在水貂白尾鹿和仓鼠身上传播。具有不同特征的新病毒变体 不断出现，具有不同的特征，在某些情况下，这些变体能够 感染原本不敏感的动物因此，持续的监视 通过Vet-LIRN进行检测对于早期识别动物内的传播至关重要。考虑到那个同伴 如果SARS-CoV-2获得传播能力， 在宠物之间或宠物和人类之间， 对野生动物种群的影响。本提案的目的是评估和验证 新的测试方法，包括验证环介导等温扩增（LAMP） 在狗和猫的各种样品基质中，并对 市售ELISA试剂盒。我们的具体目标包括：目标1：评估 商业化的比色LAMP方法用于定性检测4种新的SARS-CoV-2 RNA 从狗和猫收集的基质。目的2：使用市售ELISA检测 犬和猫的SARS-CoV-2抗体，同时建设开展SARS-CoV-2研究的能力 血清学检测这些目标将提供技术人员培训、设备和专门知识， 提供在狗和猫身上进行SARS-CoV-2测试的能力。

Project Summary Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a zoonotic disease that, in addition to its importance in the human pandemic, has caused spillover infection and onward spread in mink, white-tailed deer, and hamsters. New viral variants with different characteristics are constantly emerging, with different characteristics and in some cases, these variants are able to infect species of animals that were not originally susceptible. Therefore, ongoing surveillance through Vet-LIRN is essential for early recognition of spread within animals. Given that companion animals reside in 85 million US households, if SARS-CoV-2 were to gain the ability to spread amongst pets or between pets and humans, there could be immense public health consequence and consequence to wildlife populations. The objective of this proposal is to evaluate and validate novel testing methodologies including validating loop-mediated isothermal amplification (LAMP) in a variety of sample matrices in dogs and cats, and to perform rigorous evaluation of a commercially available ELISA kit. Our specific aims include: Aim 1: Assess the validity of a commercial colorimetric LAMP assay for the qualitative detection of SARS-CoV-2 RNA in 4 novel matrices collected from both dogs and cats. Aim 2: Validate a commercially available ELISA for SARS-CoV-2 antibodies in dogs and cats while building capacity to conduct SARS-CoV-2 serological testing. These aims will provide technician training, equipment, and expertise to provide capacity for SARS-CoV-2 testing in dogs and cats.