Histology and Imaging Core

在COBRE启动之前，新英格兰大学（UNE）没有研究核心。1期和 2 COBRE的支持导致组织学和成像核心（HIC）发展成为一个关键资源， COBRE调查人员和UNE和该地区更广泛的研究界。The Core的组织学仪器 包括组织包埋设备、切片机、振动切片机、低温恒温器和载玻片染色工作站。成像 设备包括明场、宽场荧光、载玻片扫描、共聚焦和双光子显微镜。技术 服务包括组织包埋、切片、常规组织学染色、免疫组织化学和抗体 优化和验证。在第二阶段，生境中心向38名调查员提供了服务（15名UNE-COBRE，8名UNE-非 COBRE，10名来自外部学术界，5名来自工业界），并培训了175名实验室人员。在第二阶段中， 启动了服务模式，并与布朗大学和缅因州医疗机构的其他IDEA机构建立了强有力的合作伙伴关系 中心研究所开发的，与内部用户相结合，有潜力是长期的 提供核心收入。特别注意确保核心能力不重复 由其他COBRE和INBRE-supported研究核心提供。相反，核心在战略上专门从事 独特的服务，如大脑样本，骨骼样本和海洋衍生样本的处理。在第三阶段，HIC将 保持其目前出色的业绩水平，严格评估其业绩，继续发展其人员 专业，并聘请额外的技术人员，以跟上其不断扩大的用户群的需求。HIC将 还进行了严格的战略规划方案的指导下，UNE专家在小企业战略， 完成向授标后可持续性过渡的目标。HIC已经获得了关键的额外机构 支持，包括保证关键核心人员在第3阶段后的工资、受保护的主任时间、实验室 空间，协助维护和服务合同，以及内部资助的核心凭证计划和延续 UNE小额资助计划的一部分，为那些争取校外支持的人提供资助。通过这些努力，UNE HIC将 继续为其众多用户提供出色的关键研究支持，确保UNE和 更广泛的研究社区在第三阶段及以后。

Prior to the initiation of this COBRE, there were no research cores at the University of New England (UNE). Phase 1 and 2 COBRE support resulted in the development of the Histology and Imaging Core (HIC) into a critical resource for COBRE investigators and the broader research community at UNE and the region. The Core's histology instrumentation includes tissue embedding equipment, microtomes, vibratomes, cryostats and slide staining workstations. Imaging equipment includes brightfield, widefield fluorescence, slide-scanning, confocal and 2-photon microscopes. Technical services include tissue embedding, sectioning, conventional histology stains, immunohistochemistry, and antibody optimization and validation. In Phase 2, the HIC provided services to 38 investigators (15 UNE-COBRE, 8 UNE-non- COBRE, 10 in external academia and 5 in industry), and trained 175 laboratory personnel. Also in Phase 2, a fee-for- service model was initiated, and strong partnerships with other IDeA institutions at Brown University and Maine Medical Center Research Institute were developed that, in combination with internal users, have the potential to be long-term sustaining providers of Core income. Special care has been taken to ensure that the Core capabilities do not duplicate those offered by other COBRE- and INBRE-supported research cores. Instead, the Core has strategically specialized in unique services such the processing of brain samples, bone samples and marine-derived samples. In Phase 3, the HIC will maintain its current outstanding level of performance, critically assess its performance, continue to develop its personnel professionally, and hire an additional technician to keep pace with the needs of its broadening user base. The HIC will also undertake a rigorous strategic planning program under the direction of a UNE expert in small business strategy, with the goal of completing its transition to post-award sustainability. The HIC has secured critical additional institutional support, including guarantees of post-Phase 3 salary for key Core personnel, protected time for Directors, laboratory space, assistance with maintenance and service contracts, and an internally funded core voucher program and continuation of the UNE mini-grant program for those striving for extramural support. Through these efforts, the UNE HIC will continue to provide excellent critical research support to its many users, ensuring maximum utility to UNE and the broader research community in Phase 3 and beyond.