Trapped Ion Mobility Spectrometry Quadrupole Time-of-Flight System

项目摘要 俄勒冈州州立大学正在申请资金，以增加一个捕获离子迁移谱仪 具有MALDI成像能力的四极杆飞行时间（timsTOF）LC-MS系统， 在俄勒冈州州立大学（OSU）和俄勒冈州进行生物医学研究。新 仪器将催化俄勒冈州立大学的“一个健康”研究计划，促进合作， 在人类，动物和环境健康的跨学科研究，以增加我们的知识 人类疾病的根本原因，有助于发现新的诊断标志物 创新疗法。提出的布鲁克timsTOF flexX MS系统将允许俄勒冈州的 研究社区-第一次-进行MALDI成像质谱和空间 “组学”，以加快与“一个健康”模式有关的正在进行的项目的进展。的 所要求的设备将通过提供以下途径来提高小分子组学的能力： 高分辨率离子迁移率质谱和CCS测定， 鉴定和4D“组学”（RT、mass、MS 2和CCS）。提高测量灵敏度， 先进的timsPASEF采集模式将使基于纳米级LC-MS的“组学”， 有限样本和激光捕获显微切割组织。创新的HESI源将 提高我们提供全面血浆脂质组学的能力。拟用设备 将取代俄勒冈州立大学质谱中心（OSU MSC）已有12年历史的qTOF系统。述OSU 自1973年以来，MSC提供了一个合作研究和包容性培训环境。作为 作为大学的核心设施，OSU MSC得到了研究办公室的机构支持， 多个学院，这确保了高水平的财务和运营稳定性。关于MSC 由终身教职的教师指导。两名全职员工确保高水平的 设备维护和操作的技术能力和一致性， 相关的工作流程和数据管理。研究助理为研究提供支持 项目，并协助培训学生和用户。内部监督委员会将 协调所需设备的使用和政策的管理。研究课题包括 代谢性疾病、血管性认知障碍和神经退行性疾病、植物性 补充剂，以促进衰老和斑马鱼作为毒理学的整个系统模型的恢复力。 试点和可行性计划将促进新的测量能力和MALDI成像 质谱分析法来新设备将加强全州范围内的重大研究工作， 俄勒冈州的大学、中心和机构，以协调和促进机构间的研究 以及生物医学科学方面的培训。

Project Summary Oregon State University is requesting funds for adding a trapped ion mobility spectrometry quadrupole time-of-flight (timsTOF) LC-MS system with MALDI imaging capability to advance biomedical research at Oregon State University (OSU) and in the State of Oregon. The new instrument will catalyze OSU's “ONE Health” research initiative, promoting collaborative and trans-disciplinary research in human, animal and environmental health to increase our knowledge of the underlying causes of human diseases, contribute to discovery of new diagnostic markers and innovative therapies. The proposed Bruker timsTOF fleX MS system will allow Oregon's research community - for the first time - to conduct MALDI imaging mass spectrometry and spatial “omics” to accelerate progress in ongoing projects related to the “One Health” paradigm. The requested equipment will enhance capabilities in small molecule omics by providing access to high-resolution ion mobility mass spectrometry and CCS determination, enabling compound identification and 4D “omics” (RT, mass, MS2 and CCS). Increased measurement sensitivity and the advanced timsPASEF acquisition mode will enable nano-scale LC-MS based “omics” for amount-limited samples and laser-capture microdissected tissues. An innovative HESI source will advance our capabilities to provide comprehensive plasma lipidomics. The proposed equipment will replace 12-year old qTOF systems in OSU's mass spectrometry center (OSU MSC). The OSU MSC has provided a collaborative research and inclusive training environment since 1973. As university core facility, the OSU MSC has institutional support from the research office and multiple colleges, which ensures a high level of financial and operational stability. The OSU MSC is directed by faculty with indefinite tenure. Two full time employees ensure a high level of technical competency and consistency in the maintenance and operation of the equipment, associated workflows and data management. Research associates provide support to research projects and assist in training of students and users. An internal oversight committee will coordinate use of the requested equipment and administration of policies. Research topics include metabolic diseases, vascular cognitive impairment and neurodegenerative diseases, botanical supplements to promote resilience in aging and zebrafish as whole system model in toxicology. A pilot and feasibility program will promote the new measurement capabilities and MALDI imaging mass spectrometry. The new equipment will enhance statewide efforts of the major research universities, centers and institutions in Oregon to coordinate and foster inter-institutional research and training in the biomedical sciences.