San Diego Nathan Shock Center

摘要 而衰老的特征是渐进性的、系统性的、生理性的和细胞性的衰退，其程度 这些情况的发生是高度不同的，特别是在老年人中。这份SAN补充资料的目标是 迭戈·内森休克中心(SD-NSC)正在为老龄化研究社区创造新的研究机会 人类老龄化的异质性。SD-NSC生成新的工具、资源和培训机会，以 使研究人类老龄化的异质性成为可能。这些活动的核心是人类细胞模型 老龄化核心，目前正在招募代表成人的新的人类临床队列(SD-NSC-CC) 生命线(20-85岁以上)。我们建议通过从以下方面招募老年人参与者来增强SD-NSC-CC 兰乔·贝尔纳多研究(RBS)是美国国立卫生研究院最长的基于人群的观察队列之一，包括 纵向临床评估。到目前为止，苏格兰皇家银行的参与者已经接受了多达7次身体和认知 28年来的评估，使我们能够招募代表健康的不同轨迹的苏格兰皇家银行参与者 衰老。本附录的具体目的是：1)提供生物样本和数据资源，以使 研究人员确定年龄依赖的健康轨迹如何与异质性衰老有关，2)促进 与苏格兰皇家银行的纵向数据相关联的新的人体细胞衰老模型的开发，以及3) 利用现有的SD-NSC基础设施向老龄化研究宣传这些新资源的可用性 社区。我们将共同加入SD-NSC-CC，40名苏格兰皇家银行参与者(≥65岁)代表一个范围 长期老龄化轨迹(即生物年龄)，包括对长期身体状况的复原力和易感性 和认知能力下降。将收集成纤维细胞、血浆/血清和血细胞，并将其储存和制造 可供衰老生物学研究界使用。老化核心的人体细胞模型也产生了 IPSCs和其他诱导的细胞类型(如神经元)来自成纤维细胞，维持细胞衰老的特征 捐赠者。通过将已知认知和身体功能轨迹的RBS患者纳入这一管道，我们将 显著增加了SD-NSC生成的细胞模型库，从而增加了 苏格兰皇家银行和SD-NSC都是为研究界提供的。目前的SD-NSC-CC认知电池也将 扩展到与苏格兰皇家银行认知评估相匹配，以便能够收集新的纵向认知数据。 最后，SD-NSC提供试点资金以支持老龄化研究项目的异质性，范围 它将扩大到包括那些使用这些新资源的人。总而言之，这份副刊将会成倍增加 SD-NSC和RBS的价值并增强衰老生物学研究所需的资源 社会了解人类老龄化的异质性。

ABSTRACT While aging is characterized by progressive, systemic, physiological, and cellular declines, the degree to which these occur is highly heterogeneous, particularly among older adults. The goal of this supplement to the San Diego Nathan Shock Center (SD-NSC) is to create new opportunities for the aging research community study the heterogeneity of human aging. The SD-NSC generates novel tools, resources, and training opportunities to enable studies into the heterogeneity of human aging. Central to these activities is the Human Cell Models of Aging Core that is currently enrolling a new human clinical cohort (the SD-NSC-CC) representative of the adult lifecourse (20-85+ years old). We propose to enhance the SD-NSC-CC by enrolling older adult participants from the Rancho Bernardo Study (RBS), one of NIH’s longest population-based observational cohorts that includes longitudinal clinical assessments. To date, RBS participants have undergone up to seven physical and cognitive assessments over 28 years, allowing us to enroll RBS participants representing different trajectories of healthy aging. The specific aims of this supplement are: 1) to provide biological samples and data resources to enable researchers to determine how age-dependent health trajectories relate to the heterogeneity aging, 2) to facilitate the development of novel human cell models of aging that are linked to longitudinal data from the RBS, and 3) to utilize existing SD-NSC infrastructure to advertise the availability of these new resources to the aging research communities. We will co-enroll into the SD-NSC-CC, 40 RBS participants (≥ 65 years old) representing a range of long-term aging trajectories (i.e. biological ages), including resilience and susceptibility to long term physical and cognitive decline. Fibroblasts, plasma/serum, and blood cells will be collected and banked and made available to the biology of aging research community. The Human Cell Models of Aging Core also generates iPSCs and other induced cell types (e.g., neurons) from fibroblasts that maintain cellular aging features of the donor. By including RBS patients with known cognitive and physical function trajectories in this pipeline, we will significantly augment the library of cell models being generated by the SD-NSC, thus increasing the impact of both the RBS and SD-NSC for the research community. The current SD-NSC-CC cognitive battery will also be expanded to match RBS cognitive assessments, to enable the collection of new longitudinal cognitive data. Finally, the SD-NSC provides pilot funding to support heterogeneity of aging research projects, the scope of which will be expanded to include those using these new resources. In summary, this supplement will multiply the value of both the SD-NSC and the RBS and enhance the resources needed by the biology of aging research community to understand the heterogeneity of human aging.