Structure, Composition, & Histology Core - Core B

密歇根州综合肌肉骨骼健康核心中心-核心B 项目摘要 跨生理长度尺度的结构和组成是连接基因的关键中间性状 表达与功能结果有关。影响基因表达的遗传和/或环境扰动 通常导致这些中间性状的可测量变化，这些中间性状具有机械和其他功能 后果。因此，准确量化不同长度尺度下的结构和组成是至关重要的 促进我们对肌肉骨骼健康背后的分子和力学机制的理解 和疾病；制定和评估治疗策略；并最终将这些策略转化为 诊所。然而，这项研究需要使用先进而昂贵的仪器和细胞方面的辅助专业知识 生物、化学、工程和生物医学成像。结构、成分和组织学核心 (核心B)将提供使用工具和专业知识的途径，使中心调查人员能够量化 这些中间性状并加快了他们的研究计划。核心B将包括体外微型计算机 断层扫描(µCT)和纳米计算机断层扫描(Nano-CT)、拉曼光谱和组织学作为其 主要组成部分。选择这些技术是因为它们适用于所有肌肉骨骼组织和 基于过去4年的使用数据和对核心用户的需求评估调查。调查人员在 密歇根大学率先使用微CT和拉曼光谱研究肌肉骨骼 该公司是应用这些技术解决肌肉骨骼问题的领先者。组织学继续 是了解组织形态变化的门户方法，当与CT和 拉曼光谱，组织学可以将组织形态与成分和功能结果联系起来。目标 这个核心的核心是利用核心领导层的专业知识来支持中心的研究计划 对所有肌肉骨骼组织进行结构、成分和组织学分析的研究人员， 在智力和经济上；为我们的研究界扩大机会；并带来新的研究人员 进入肌肉骨骼社区。实现我们的目标，继续创造一个强大和可持续的 对于P30更新申请对肌肉骨骼社区的影响，我们提出了以下具体目标： 目标1：提供关于最佳利用技术和实验设计的专门知识和指导，以使 研究人员进行专门的分析，以量化不同长度尺度的结构和组成。 目标2：提供最新技术方面的专家使用、标准化和培训，以量化结构 以及跨越生理长度尺度的成分。 目标3：提供数据分析、结果解释和数据分析方面的专门知识和科学指导 对未来研究的建议。 目标4：通过创新和发展赋能技术，为我们的研究界扩大机会 技术、教育、培训和丰富。

Michigan Integrative Musculoskeletal Health Core Center – Core B Project Summary Structure and composition across physiological length-scales are key intermediate traits that link gene expression with functional outcomes. Genetic and/or environmental perturbations affecting gene expression often lead to measureable changes in these intermediate traits that have mechanical and other functional consequences. Accurately quantifying structure and composition at different length scales is therefore essential to advancing our understanding of the molecular and mechanical mechanisms underlying musculoskeletal health and disease; to develop and evaluate treatment strategies; and to ultimately translate these strategies to the clinic. However, this research requires use of advanced and costly instruments and supporting expertise in cell biology, chemistry, engineering, and biomedical imaging. The Structure, Composition and Histology Core (Core B) will provide access to the instruments and expertise that will enable Center investigators to quantify these intermediate traits and to accelerate their research programs. Core B will include ex-vivo micro-Computed Tomography (µCT) and nano-Computed Tomography (nano-CT), Raman spectroscopy, and histology as its major components. These technologies were chosen for their applicability to all musculoskeletal tissues and based on usage data over the previous 4 years and a needs-assessment survey of core users. Investigators at the University of Michigan pioneered the use of µCT and Raman spectroscopy to investigate musculoskeletal tissues and are leaders in applying these technologies to solve musculoskeletal problems. Histology continues to be the gateway approach to understanding changes in tissue morphology and, when combined with CT and Raman spectroscopy, histology can link tissue morphology to composition and functional outcomes. The goals of this Core are to leverage the expertise of the core leadership to support the research programs of Center investigators in performing structural, compositional and histological analyses of all musculoskeletal tissues, both intellectually and financially; to expand opportunities for our research community; and to bring new investigators into the musculoskeletal community. To accomplish our goals and to continue creating a robust and sustainable impact to the musculoskeletal community in the P30 renewal application, we propose the following Specific Aims: Aim 1: To provide expertise and guidance on the best use of technologies and experimental design to allow investigators to conduct specialized assays for quantifying structure and composition across length scales. Aim 2: To provide expert use, standardization, and training in state-of-the-art technologies to quantify structure and composition across physiological length scales. Aim 3: To provide expertise and scientific guidance on data analysis, interpretation of results and recommendations for future studies. Aim 4: To expand opportunities for our research community through innovation and development of enabling technologies, education, training, and enrichment.