Integrated Structural Biology Core

生物分子结构与动力学中心的综合结构生物学核心(ISBC)设施 (CBSD)提供技术和专业知识，帮助蒙大拿大学(UM)的教职员工阐明生物学 使用机械和结构生物化学的分析方法的过程。所提供的服务 核心包括一整套基因到结构技术：蛋白质表达和纯化、结构 用X射线结晶学和低温电子显微镜(Cryo-EM)进行测定。ISBC有能力 进行各种蛋白质热力学和配基结合的生物物理和分析测量 具有表面等离子激元共振和多角度光散射等特性，并支持位于 用于大分子结构和动力学的结构分析，以及小型有机化合物的结构验证 分子。ISBC还成为蒙大拿州和 周边落基山脉地区，包括三个理想之州。在Cobre支持的第三阶段期间 ISBC寻求：1)保持结构生物学方法论的前沿，除其他活动外， 与蒙大拿州立大学的合作伙伴合作，发展低温EM服务的能力；2) 与CBSD和其他Cobre Core内部的其他核心设施保持密切和持续的互动 UM的设施提供一套互补的实验和分析工具来解决复杂的问题 分子和细胞生物学方面的问题；3)优化ISBC报告流程，有效跟踪服务 提供给客户，评估科学成果和影响，并监测其财务状况。ISBC将 使用评估/调查工具来确保客户满意度，识别新出现的需求，并定义清晰和 透明的机制来确定项目的优先顺序。ISBC将继续执行其培训任务，以 向客户实验室传播技术。最后，ISBC将实施政策，以确保其 通过保持强大的按服务收费计划，利用机构支持，实现第三阶段以外的可持续性 以及寻求与UM调查人员和外部用户进行赠款资助的合作。

The Integrated Structural Biology Core (ISBC) facility of the Center for Biomolecular Structure and Dynamics (CBSD) provides technology and expertise to assist University of Montana (UM) faculty to elucidate biological processes using the analytical methods of mechanistic and structural biochemistry. Services provided by the Core include a full suite of gene-to-structure technologies: protein expression and purification, structure determination by X-ray crystallography and cryo-Electron Microscopy (cryo-EM). The ISBC has the capacity to conduct a variety of biophysical and analytical measurements of protein thermodynamic and ligand-binding properties such as surface plasmon resonance and multi-angle light scattering, and supports NMR facilities at UM for structural analysis of macromolecular structure and dynamics, and structural validation of small organic molecules. The ISBC has also become a facility for small molecule X-ray crystallography for Montana and the surrounding Rocky Mountain region, which include three IDeA States. During Phase III of COBRE support the ISBC seeks: 1) to remain at the methodological forefront of structural biology by, among other activities, developing capacity for cryo-EM services in collaboration with partners at Montana State University; 2) to maintain strong and ongoing interactions with other core facilities within CBSD and other COBRE Core facilities at UM to provide a suite of complementary experimental and analytical tools to solve complex problems in molecular and cell biology; 3) optimize ISBC reporting processes to effectively track services provided to clients, evaluate scientific outcomes and impact, and monitor its financial status. The ISBC will employ evaluation/survey tools to ensure client satisfaction, identify emerging needs and define clear and transparent mechanisms to prioritize projects. The ISBC will continue to pursue its training mission to disseminate technologies to client laboratories. Lastly, the ISBC will implement policies to insure its sustainability beyond Phase III by maintaining a robust fee-for-service program, leveraging institutional support and pursuing grant-funded collaborations with UM investigators and external users.