BRAIN Initiative K99 Project

根据该提案的目的，进行了以下实验，以确定伏隔核(NAC)中含有多巴胺D2受体(D2R)的神经元在食物摄入中的作用： 1-评估纹状体D2R低的动物寻找和消费食物的动机。在靶向缺失drd2和产仔对照的小鼠中使用可操控食物自我给药的行为实验。行为经济学分析使用递进比率响应增加获得单一食物颗粒(报酬)所需的努力，使我们能够计算成本-收益函数。实验仍在进行中。初步数据显示，动机的变化与纹状体中低水平的D2R有关。 2.低D2R组和产仔对照组小鼠脑片中诱发多巴胺信号的测定。我们正在评估胰岛素对多巴胺信号的影响，以及当D2R水平降低时胰岛素效应的变化。

In accordance with aims of the proposal, the following experiments were done to determine the role of dopamine D2 receptor (D2R) containing neurons in the nucleus accumbens (NAc) in food intake: 1- Assessing motivation to seek and consume food in animals with low D2R in the striatum. Behavioral experiments using operant food self-administration in mice with targeted deletion of Drd2 and littermate controls. Behavioral economic analysis using progressive ratio responding to increase the effort required to obtain a single food pellet (reward) allows us to calculate the cost-benefit function. Experiments are still underway. Preliminary data shows changes in motivation associated with low levels of D2R in the striatum. 2. Measurements of evoked dopamine signals in brain slices from mice with low D2R and littermate controls. We are evaluating the effect of insulin on the dopamine signals and the changes in the insulin effect when D2R levels are decreased.