Administrative supplement to purchase a MerMade 4 oligonucleotide synthesizer for the large-scale production of modified RNA substrates

摘要： 我们的NIH R35 ESI Mira题为‘铁对mRNA去甲基化的选择性和调节-- 依赖双加氧酶的研究重点在于了解细胞的结构和分子决定因素 ALKBH家族RNA去甲基酶，在RNA功能和某些人类中起关键作用 癌症。进行一些拟议的结构实验面临的一个关键挑战是 一直在获得足够数量的修饰RNA寡核苷酸底物，用于 结晶学筛选和生物物理结合分析。本行政副刊 申请资金购买LGC MerMade 4寡核苷酸合成器，将改变 并极大地提高了我们获取关键修饰RNA寡核苷酸试剂的能力 我们Mira项目的多个目标。

ABSTRACT: Our NIH R35 ESI MIRA entitled ‘Selectivity and regulation of mRNA demethylation by iron- dependent dioxygenases’ focuses on understanding the structural and molecular determinants of AlkBH-family RNA demethylase enzymes that play key roles in RNA function and some human cancers. A critical challenge to carrying out some of the proposed structural experiments has been in obtaining sufficient quantities of modified RNA oligonucleotide substrates for crystallographic screening and biophysical binding assays. This administrative supplement requests funds to purchase an LGC MerMade 4 oligonucleotide synthesizer that will transform and greatly accelerate our ability to access key modified RNA oligonucleotide reagents to support multiple goals of our MIRA projects.