PHOTOCLEAVABLE LINKERS AND MARKERS FOR MASS SPECTROMETRY

描述：（改编自申请人的摘要） 项目是开发一种新的技术，用于检测和分析 基于新型光可裂解连接体（PC-连接体）的生物分子， 光可裂解标记物（PC标记物）和基质辅助激光解吸 质谱（MALDI-MS）。 AmberGen公司 已经证明 生物分子可以使用PC连接体捕获在表面上，然后 光释放并使用MALDI-MS检测。 通过MALDI-MS使用含有PC-的探针以高灵敏度检测。 标记。 将PC-连接体和PC-标记物引入生物分子中 使用AmberGen，Inc. 实例包括 用于寡核苷酸的5 '-末端标记的PC-亚磷酰胺 固相支持合成和PC-NHS-酯用于标记蛋白质， 多肽。 在第一阶段，这项新技术将由 将PC-接头和PC-标记物并入多种生物分子中， 包括核酸、蛋白质和多肽， 通过MALDI-MS分析分子。 含有PC-连接物和PC-标记物的生物分子的2-D图案化阵列 将使用MALDI-MS产生和评价。PC-连接物和PC-标记物 与MALDI-MS结合在分子诊断中有许多应用 如DNA分析、新生蛋白分析和药物筛选。

DESCRIPTION: (Adapted from the applicant's abstract) The goal of this project is to develop a new technology for the detection and analysis of biomolecules based on novel photocleavable linkers (PC-linkers), photocleavable-markers (PC-markers) and matrix assisted laser desorption mass spectrometry (MALDI-MS). AmberGen, Inc. has demonstrated that biomolecules can be captured on a surface using PC-linkers and then photreleased and detected using MALDI-MS. Biomolecules can also be detected with high sensitivity by MALDI-MS using probes which contain PC- markers. PC-linkers and PC-markers are introduced into biomolecules using proprietary reagents developed by AmberGen, Inc. Examples include PC-phosphoramidites for 5'-end labeling of an oligonucleotide during solid-support synthesis and PC-NHS-esters for labeling of proteins and polypeptides. During Phase I this new technology will be evaluated by incorporating PC-linkers and PC-markers into a variety of biomolecules including nucleic acids, proteins and polypeptides and analyzing these molecules by MALDI-MS. Substrates containing uniform and microfabricated 2-D patterned arrays of biomolecules containing PC-linkers and PC-markers will be produced and evaluated using MALDI-MS. PC-linkers and PC-markers combined with MALDI-MS have numerous applications for molecular diagnosis such as DNA analysis, nascent protein analysis and drug screening.