60 HZ MAGNETIC FIELD INDUCED DNA DAMAGE

我们实验室最近的研究表明，大鼠急性(2小时) 暴露在60赫兹磁场(0.1-0.5mT)中会增加 他们脑细胞中的DNA单链和双链断裂。实验 建议进一步研究暴露于 60赫兹的磁场会导致大鼠脑细胞的DNA损伤。 具体地说，将调查自由基所起的作用， 因为我们的初步研究还表明，之前对老鼠的治疗 接触自由基清除剂(褪黑素和自旋陷阱 化合物)可以阻断磁场对DNA的破坏作用。免费 最近，自由基被认为与生物效应有关。 极低频(ELF)磁场。设计了实验 为了研究自由基在磁场中的参与- 诱导细胞内DNA链断裂。这项拟议的研究具有 以下是具体目标： (1)进一步证实褪黑素的磁封闭作用 电场诱导小鼠脑细胞DNA单链和双链断裂 大鼠，将进行(药物)剂量反应研究。 (2)进一步支持自由基是 参与磁场效应的动物，会被用来治疗 不同的自旋陷阱化合物和维生素E类似物曲洛克斯 研究它们是否能阻止磁场对DNA的影响 大鼠脑细胞中的链断裂。自旋陷阱化合物和trolox是 高效的自由基清除剂。 这是极低频电磁场研究的一个重要新方向。 生物效应研究，因为DNA损伤与人类健康密切相关 风险。特别是，脑细胞中的DNA损伤可能会影响神经 功能，也可能导致致癌和神经退行性变 疾病。希望来自这项拟议研究的数据将有所帮助 在确定接触权力的可能因果关系方面 频率磁场和生物效应也在危害中 工频电磁场的评估。

Recent research in our laboratory shows that rats acutely (2 hrs) exposure to a 60-Hz magnetic field (0.1 - 0.5 mT) have an increase in DNA single and double strand breaks in their brain cells. Experiments are proposed to further investigate the mechanism by which exposure to a 60 Hz magnetic field causes DNA damages in brain cells of the rat. Specifically, the role played by free radicals will be investigated, since our preliminary research also shows that treatment of rats before exposure with free radical scavengers (melatonin and a spin trap compound) can block the DNA damaging effect of magnetic fields. Free radicals have recently been implicated in the biological effects of extremely low frequency (ELF) magnetic fields. Experiments are designed to investigate the involvement of free radicals in magnetic field- induced DNA strand breaks in cells. This proposed research has the following specific aims: (1) To further confirm the blocking effect of melatonin on magnetic field-induced DNA single and double strand breaks in brain cells of rats, a (drug) dose-response study will be carried out. (2) To provide further support for the hypothesis that free radicals are involved in the effect of magnetic fields, animals will be treated with different spin-trap compounds and the vitamin E analog trolox to investigate whether they can block the effects of magnetic fields on DNA strand breaks in rat brain cells. Spin-trap compounds and trolox are efficient free radical scavengers. This is an important new direction in ELF electromagnetic field bioeffect research because DNA damage is closely related to human health risk. Particularly, DNA damage in brain cells could affect neurological functions and also possibly lead to carcinogenesis and neurodegenerative diseases. It is hoped that data from this proposed research will help in identifying possible causal connections of exposure to power frequency magnetic fields and biological effects and also in the hazard assessment of power frequency electromagnetic fields.