CONTINUING STUDY ON AIDS-RELATED OPPORTUNISTIC PATHOGENS

描述：（改编自研究者摘要。）主 该提案的目的是继续进行已经建立的研究 William巴罗博士（PI），南方研究 巴斯德研究所的Nalin Rastogi博士（共同PI）。的 合作开始时，巴罗博士担任休假博士。 Rastogi的实验室作为Fogarty Senior International的一部分 1992年、1994年研究金。该研究金的目的是 更好地了解鸟分枝杆菌复合体， 在致病性、毒力和多重耐药性方面。那些 研究被设计为与两个NIH赠款互动， 这是PI研究计划的一部分，因此在 继续为这些赠款提供资金。此外，还有几份手稿， 这是这些研究成果的成果。拉斯托吉博士一直在 在巴黎的巴斯德研究所工作了十多年。去年 他搬到了法国瓜德罗普岛的巴斯德研究所， 他目前是结核病和分枝杆菌科科长 单位由于这一举措，与拉斯托吉博士的合作 为涉及艾滋病相关的扩展研究提供了独特的环境 病原体如M. avium和M.结核这是特别 重要的是，当人们考虑多药耐药（MDR）的影响时， 菌株M.结核病和早期发现的重要性， 监测这些菌株的发展。另一组 对HIV+个体构成威胁的分枝杆菌是M.鸟 复杂，这不仅是耐多种药物，但这不是 关于它的致病性我们已经很清楚了。作为数字的 艾滋病毒阳性个体在全球范围内增加，特别重要的是， 建立一个全球监控系统， M.结核病，并评估新的方法， 治疗MDR菌株不仅M.结核病，但M. Avium Complex也是。本提案的具体目标是：1） 分离和鉴定M. avium和M.结核型 瓜德罗普艾滋病毒阳性和阴性患者 2）获得药物敏感性模式， 每个菌株，和3）表征M. avium和M.结核 通过限制性片段长度多态性， 致病性和耐药性。此次合作不仅将 允许PI与M. avium，但也让他扩大他的研究工作，以另一个 重要的AIDS相关病原体，M.结核

DESCRIPTION: (Adapted from the Investigator's Abstract.) The primary goal of this proposal is to continue an already established research collaboration between Dr. William Barrow (PI), Southern Research Institute, and Dr. Nalin Rastogi (Co PI) of the Institut Pasteur. The collaboration began when Dr. Barrow served a sabbatical leave in Dr. Rastogi's laboratory as a part of a Fogarty Senior International Fellowship from 1992, 1994. The purpose of that fellowship was to develop a better understanding of the Mycobacterium avium complex with regard to pathogenicity, virulence and multiple drug resistance. Those studies were designed as interactive with two NIH grants that were a part of the PI's research program, and as a result were important in the continued funding of those grants. In addition, several manuscripts were published as a result of those research efforts. Dr. Rastogi has been with the Institut Pasteur in Paris for more than ten years. Last year he moved to the Institut Pasteur on the island of Guadeloupe, France, where he is currently the Chief of the Tuberculosis and Mycobacteria Unit. As a result of that move, the collaboration with Dr. Rastogi offers a unique situation for extended studies involving AIDS related pathogens such as M. avium and M. tuberculosis. This is particularly important when one considers the impact of multiple drug resistant (MDR) strains of M. tuberculosis and the importance of early detection and monitoring of these strains once they develop. Another group of mycobacteria that poses a threat to HIV+ individuals is the M. avium complex, which is not only resistant to multiple drugs, but which is not well understood with regard to its pathogenicity. As the numbers of HIV+ individuals increases worldwide, it is particularly important to develop a global surveillance system for the isolation and containment of MDR strains of M. tuberculosis, and to evaluate new methods for treatment of MDR strains of not only M. tuberculosis but those of the M. avium complex as well. The specific aims of this proposal will be to 1) isolate and characterize strains of M. avium and M. tuberculosis form HIV positive and HIV negative patients in Guadeloupe and the surrounding Caribbean area, 2) obtain drug susceptibility patterns for each strain, and 3) characterize strains of M. avium and M. tuberculosis by restriction fragment length polymorphism for further studies on pathogenicity and drug resistance. This collaboration will not only allow the PI to enhance his current NIH funded research efforts with M. avium but also allow him to expand his research efforts to another important AID related pathogen, M. tuberculosis.