REGULATION OF K+ TRANSPORT IN VASCULAR SMOOTH MUSCLE

这一建议的主题是收缩之间的关系， 血管平滑肌和跨膜钾转运的同时变化 细胞膜。 特别是，这次调查的重点是 负责钾增加的特定离子运输过程 当平滑肌细胞受到刺激时， 收缩。 初步数据表明，这种离子的大部分 运输不通过离子通道发生。 相反，主要的 钾流出途径依赖于氯的存在， 被髓袢利尿剂抑制 这两个特征有力地证明， 该途径代表某种形式的共转运。 研究以确定和 描述了这种运输系统的特点。 功能重要性 将通过评估中断是否 限制了动脉收缩的能力。 初步 研究还表明，在细胞内， 钙和钾转运过程的激活。 因此，最后 本研究的第一阶段将研究细胞内 钙和钾转运过程的激活。 因此，最后 本研究的第一阶段将研究细胞内 钙浓度和共转运系统的活性。 这 目的包括评价培养的 血管平滑肌细胞 实现这一目标将使 对这种离子运输的调节进行更详细的研究的阶段 系统 这些研究之所以重要和相关，主要有两个原因。 首先，这项调查将促进我们了解如何单价 离子转运系统参与血管系统的调节。 第二，这些研究对我们的经济发展有着直接和直接的影响。 对高血压和其他疾病治疗的理解和原理 心血管疾病过程。

The subject of this proposal is the relationship between contraction of vascular smooth muscle and concurrent changes in potassium transport across the cell membrane. In particular, this investigation focuses on the specific ion transport processes responsible for the increase in potassium and chloride transport out of smooth muscle cells when they are stimulated to contract. The preliminary data indicate that the majority of this ion transport does not occur via ion channels. Instead, the predominant pathway for potassium efflux is dependent on the presence of chloride and inhibited by loop diuretics. These two features are strong evidence that this pathway represents some form of cotransport. Studies to identify and characterize this transport system are outlined. The functional importance of this ion transporter will be evaluated by assessing whether interruption of the system limits the ability of arteries to contract. The preliminary studies also demonstrate that there is a tight link between intracellular calcium and activation of the potassium transport process. Thus, the final phase of this study will examine the relationship between intracellular calcium and activation of the potassium transport process. Thus, the final phase of this study will examine the relationship between intracellular calcium concentration and activity of the cotransport system. This objective includes an evaluation of potassium cotransport in cultured vascular smooth muscle cells. Achievement of this objective will set the stage for more detailed studies of the regulation of this ion transport system. The studies are important and relevant for two primary reasons. First, this investigation will advance our understanding of how monovalent ion transport systems participate in regulation of the vascular system. Second, these studies have immediate and direct bearing on our understanding and rationale for treatment of hypertension and other cardiovascular disease processes.