DIETARY SOY SUPPLEMENTS: CARDIOPROTECTIVE MECHANISMS

这项提案的总体目标是确定组成部分(S) (即特定的异黄酮组分或多肽组分) 对于其对心血管系统的保护作用，评估其 相对生物活性及其作用机制的研究。以下五个方面 问题将被解决：(1)大豆蛋白的成分是什么 说明其有益的作用或致动脉粥样硬化；(2)哪种异黄酮类 (例如，染料木素或大豆苷元)有助于大豆对 血管舒缩功能与动脉粥样硬化；(3)大豆的作用依赖于 异黄酮肽或异黄酮类雌激素相互作用； 需要特定的异黄酮类和雌激素来诱导和优化这些 作用；以及(5)雌激素受体依赖和 大豆心脏保护作用的独立调节过程？至 研究大豆中多种成分的单独和联合作用 人类不易研究的动脉粥样硬化斑块的形成 受试者和非人类灵长类动物，导致基因突变的小鼠 增加动脉粥样硬化和/或动脉粥样硬化的易感性 雌激素受体缺乏症将被使用。建议的研究将 评估大豆的各个成分的作用及其可能的作用 在介导主要心脏保护作用中的交互作用 已被报告为大豆。这一结果将进一步促进人们对大豆 它具有心脏保护特性，并导致了优化其有利效果的策略。

The overall objective of this proposal is to identify the component(s) (i.e., specific isoflavones or peptide fractions) of soy that account for its protective effects on the cardiovascular system, assess their relative bioactivity and study mechanisms of action. The five following questions will be addressed: (1) what are the constituents of soy protein that account for its beneficial effects or atherogenesis; (2) which isoflavones (i.e., genistein or daidzein) contribute to soy's favorable effects on vasomotor function and atherosclerosis; (3) do the effects of soy depend on isoflavone peptide or isoflavone estrogen interactions; (4) what amounts of specific isoflavones and estrogen are needed to elicit and optimize these effects; and (5) what are the relative roles of estrogen receptor dependent and independent processes in mediating the cardioprotective effects of soy? To study the separate and combined effects of many components of soy on the development of atherosclerotic plaques, which cannot be studied easily in human subjects and nonhuman primates, mice with induced genetic mutations that result in increased susceptibility to the development of atherosclerosis and/or a deficiency in estrogen receptors will be used. The studies proposed will evaluate the role of the individual constituents of soy and their possible interactive effects in mediating the major cardioprotective effects that have been reported for soy. The results will further advance understanding of soy's cardioprotective properties and lead to strategies for optimizing its favorable effects.