Holographic Decoding of Biochemical Libraries

描述（由申请人提供）：Nanohmics开发了一个从随机有序数组中解码生化文库化合物特征的平台。该方法采用一种新颖的全息成像技术，在整个固定库的单个图像采集过程中识别与任何化合物的正反应。该方法的前提是微/纳米粒子标记补体的三维轮廓，或在模板签名模式的边界内进行分析。签名图案是在同一文库化合物的数百万份副本上生成的，使用微光刻工艺来定义图案。每个签名的单个副本从模板表面释放到能够扩散到溶液中的独立支撑结构中。支持结构的批量制造可以对库中的不同特征化合物并行进行。不同化合物的支持结构可以混合形成文库，而不需要每次构建文库时重建阵列或合成新的序列。全息成像方法最近在半导体工业中被证明用于缺陷检测。Nanohmics计划首次将数字全息成像方法应用于结合生物化学物种的分析。该技术将使超高通量筛选成为可能，因为同时解码库中所有化合物的随机有序阵列的能力提供了串行微阵列工艺的优越优势。

DESCRIPTION (provided by applicant): Nanohmics has developed a platform for decoding signatures of biochemical library compounds from randomly ordered arrays. The approach uses a novel holographic imaging technique to identify positive reactions with any compounds during a single image acquisition of the entire immobilized library. The premise for the method is three-dimensional contouring of micro/nanoparticle-labeled complements, or analyzes within the boundaries of a templated signature pattern. Signature patterns are generated on millions of copies of the same library compound using microlithographic processes to define the pattern. A single copy of each signature is liberated from the template surface into freestanding support structures able to diffuse into solution. Batch fabrication of the support structures can occur in parallel for different signature compounds in the library. The support structures for different compounds can be mixed to form libraries without the need for rebuilding the array or synthesizing new sequences each time a library is constructed. The method of holographic imaging has recently been demonstrated for defect detection in the semiconductor industry. Nanohmics plans to apply the method of digital holographic imaging for the analysis of bound biochemical species for the first time. This technique will enable ultra high throughput screening as the ability to simultaneous decode all the compounds in the library for a randomly ordered array offers superior advantage to serial microarraying processes.