Histology, Tissue Processing and High Content Microscopy

摘要 组织学、组织处理和高含量显微镜核心 组织学、组织处理和高含量显微镜核心将继续满足以下需求： UCSD视觉研究人员通过提供专用的组织处理设备和兼职 具有眼组织学和免疫组织化学经验组织技术员。组织学，组织处理 和高含量显微镜核心支持石蜡和冷冻切片处理以及各种染色 战略布局该核心还为视觉研究人员提供了1）根据以下因素选择注视点的建议： 研究目的; 2）研究样本的石蜡和冷冻封闭; 3）石蜡或冷冻组织学切片制备 定制染色，4）帮助正确解释和精确的区域微取样 5）用于药物筛选的高含量共聚焦成像， 组织学标本成像。

ABSTRACT Histology, Tissue Processing and High Content Microscopy Core The Histology, Tissue Processing and High Content Microscopy Core will continue to serve the needs of UCSD vision researchers by providing dedicated tissue processing equipment and a part-time histotechnician experienced in eye histology and immunohistochemistry. The Histology, Tissue Processing and High Content Microscopy Core supports both paraffin and cryosection processing and various staining strategies. The core also provides vision researchers with 1) advice for fixation selection depending on study purpose; 2) paraffin and cryo blocking of study samples; 3) paraffin or cryo histology slide preparation with customized staining, 4) assistance for proper interpretation and precision microsampling of the region of interest for electron microscopy evaluation and 5) high content confocal imaging for drug screening and histologic specimen imaging.