Flow Cytometry & Single Cell Core Facility (FCSCCF)

项目总结/摘要-流式细胞术和单细胞核心设施（FCSCCF） 西弗吉尼亚大学流式细胞术和单细胞核心设施（FCSCCF）的使命是提供最先进的 生物医学研究的技术、仪器和专业知识。作为肿瘤微环境的一部分 (TME)生物医学研究卓越中心（CoBRE），WVU FCSCCF扩大了其单细胞 分析服务，包括使用10 x Genomics Chromium的单细胞条形码和文库生成 控制器和全光谱3激光Cytek Aurora分析流式细胞仪。这些服务/工具 补充设施中的其他仪器，包括三个流式细胞仪（4-激光BD FACSAria III分选仪， 4-激光LSRFortessa分析仪和1-激光Guava easycyte HT），两个磁性细胞分离器（Miltenyi Biotec AutoMACS Pro和Multi-MACS 24），Fluidigm C1 Auto Prep系统，Miltenyi Biotec gentleMACS Octo 解离器、马尔文Panalytic NanoSight NS 300和ZetaSizer NanoZ。该设施开发了一个 与WVU Genomics Core和WVU Bioinformatics Core合作的强大管道， 从样品制备到数据分析的协助/专业知识的研究人员。具体目标是 研究核心应用是：1）实施和支持TME CoBRE项目的研究项目 领导者和试点项目接受者，因为他们努力获得NIH R 01资金和2）提供特殊的 为TME CoBRE-funded研究人员提供流式细胞术和单细胞分析领域的专业知识和培训 和其他机构发展奖（IDEA）资助的项目在西弗吉尼亚大学。该提案描述了服务 设施提供的培训、其管理计划、费用结构、质量保证计划和战略 在项目的整个生命周期及以后维持和扩大该设施。

PROJECT SUMMARY/ABSTRACT - Flow Cytometry and Single Cell Core Facility (FCSCCF) The mission of the WVU Flow Cytometry and Single Cell Core Facility (FCSCCF) is to provide state-of-the-art technologies, instrumentation, and expertise for biomedical research. As part of the Tumor Microenvironment (TME) Center of Biomedical Research Excellence (CoBRE), the WVU FCSCCF has expanded its single cell analysis services to include single cell barcoding and library generation using a 10x Genomics Chromium Controller and a full spectrum 3-laser Cytek Aurora analytical flow cytometer. These services/instruments complement the other instruments in the facility including three flow cytometers (4-laser BD FACSAria III sorter, 4-laser LSRFortessa analyzer, and 1-laser Guava easycyte HT), two magnetic cell separators (Miltenyi Biotec AutoMACs Pro and Multi-MACS24), a Fluidigm C1 Auto Prep System, a Miltenyi Biotec gentleMACS Octo Dissociator, a Malvern Panalytical NanoSight NS300, and a ZetaSizer NanoZ. The facility has developed a robust pipeline in collaboration with the WVU Genomics Core and WVU Bioinformatics Core to provide WVU researchers with assistance/expertise from sample prep through data analysis. The specific aims of this research core application are: 1) Implement and support the research projects of the TME CoBRE Project Leaders and Pilot Project Recipients as they work toward obtaining NIH R01 funding and 2) Provide exceptional expertise and training in the area of flow cytometry and single cell analysis to TME CoBRE-funded investigators and other Institutional Development Award (IDeA)-funded projects at WVU. The proposal describes the services and training provided by the facility, its management plan, fee structure, quality assurance plan, and strategies to sustain and expand the facility over the life of the project and beyond.