Investigations Of Macromolecular Structures And Dynamics

该实验室的总体研究目标集中在尽可能完整地描述肽，蛋白质，核酸及其复合物在溶液中的结构，主要是通过NMR光谱。目前，特别强调的是发展的方法，使调查更大和复杂的系统，以及增加精度，这些解决方案的结构可以得到。进行了旨在关联结构和功能的研究以及旨在研究蛋白质折叠的实验。已经对几种蛋白质进行了结构研究。这些包括HIV-1蛋白酶、氰毒蛋白-N、杂合硫氧还蛋白和复制起始蛋白。此外，还对模型蛋白GB 1的不同四级态的折叠进行了研究。新媒体部分排列分子在磁场中的特点和利用测量残余偶极耦合。此外，新的NMR实验，以确定这些耦合设计。

The objective of the overall research in this laboratory is centered on achieving as complete a description as possible for the structures of peptides, proteins, nucleic acids and their complexes in solution, principally by NMR spectroscopy. At present particular emphasis is being placed on developing approaches which allow the investigation of larger and complex systems as well as increase the precision with which these solution structures can be obtained. Studies aimed at correlating structure and function, and experiments aimed at investigating protein folding are conducted. Structural studies for several proteins have been carried out. These comprise HIV-1 protease, cyanovirin-N, a hybrid thioredoxin and a replication initiator protein. In addition, work was also carried out on the folding of different quaternary states of the model protein GB1. New media for partially aligning molecules in the magnetic field were characterized and exploited for measuring residual dipolar couplings. In addition, novel NMR experiments for determining these couplings were devised.