Anti-PSA single chain antibody-quantum dot conjugates

描述(由申请人提供)： 此SBIR方案旨在制备可用于PSA免疫分析的发光抗PSA(前列腺特异性抗原)单链抗体片段(ScFv)-量子点(QD)结合物。该项目将建立在我们最近成功制备发光的、单分散的、超稳定的、可溶的和生物相容的树枝状盒子半导体纳米晶的基础上，以及本实验室早期在抗PSA单链抗体片段scFvB80的克隆、表达和结构表征方面的工作。这一提议的成功可能为制备抗体-QD偶联物提供了一条新的途径。与现有的抗体-受体蛋白-量子点偶联物相比，该ScFvB80-量子点将直接偶联，具有体积小、结构简单、制备容易、生产成本低、易于通过基因工程进行修饰等优点。所提出的抗PSA单链抗体FvB80-QD偶联物具有改进PSA免疫分析的潜力。

DESCRIPTION (provided by applicant): This SBIR proposal intends to prepare luminescent anti-PSA (prostate specific antigen) single chain antibody fragment (scFv) -- Quantum Dot (QD) conjugates that can be used for PSA immunoassays. This project will be built on our recent success in preparing luminescent, monodisperse, super stable, water-soluble, and bio-compatible dendron-boxed semiconductor nanocrystals, as well as on this Pl's earlier works in the cloning, expression and structural characterization of an anti-PSA single chain antibody fragment-scFvB80. The success of this proposal could provide a new path of making antibody-QD conjugates. In comparison with current antibody-adaptor protein-QDs conjugates, this proposed scFvB80-QDs would be directly coupled and could have the advantages of small size, simple structure, easy preparation, low production costs, and ready modification via genetic engineering. The proposed anti-PSA scFvB80-QD conjugate could have the potential for improving the PSA immunoassay.