HIV AND SUBSTANCE ABUSE LABORATORY CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这一构成部分的目标是建立一个拥有所需人力和物力的核心实验室，以支持艾滋病毒/艾滋病和滥用药物领域的研究。今后五年，将向研究人员提供专门设施，用于免疫学、滥用药物、艾滋病毒/艾滋病和相关传染病等领域的科学研究。将开发一个体外系统，在受控的实验室条件下测试与成瘾相关的药物及其相互作用对艾滋病毒复制和免疫反应的影响。核心实验室将为研究人员提供核酸检测、病毒基因分型、流式细胞仪表型、淋巴细胞增殖、细胞毒性和非细胞毒性活性、细胞因子和趋化因子测定以及滥用药物定量的分析。将继续向用户提供顾问和导师，以有效开发其项目，并促进与外部研究人员的合作。此外，还要求提供支持，以改进标本储存库、实验室信息系统、与数据核心的电子通信以及遵守HIPAA和IRB条例。此外，还描述了RCMI支持的基础设施的制度化努力。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this component is to establish a core laboratory with the needed human and physical resources to support research studies in the areas of HIV/AIDS and substances of abuse. For the next five years, specialized facilities will be made available to researchers for scientific studies in fields of immunology, drugs of abuse, HIV/AIDS and related infectious diseases. An in vitro system will be developed to test for the effect of addiction-related drugs and their interaction on HIV replication and the immune response under controlled laboratory conditions. The core lab will provide researchers with assays for nucleic acids detection, virus genotyping, flow-cytometric phenotyping, lymphocyte proliferation, cytotoxic and non-cytotoxic activity, cytokine and chemokine determinations, and drugs of abuse quantification. Consultants and mentors will continue to be available to users for the effective development of their projects and foster collaboration with external researchers. Moreover, support is requested to improve specimen repository, laboratory information system, electronic communication with Data Core, and compliance with HIPAA and IRB regulations. Furthermore, an institutionalizing effort of RCMI-supported infrastructure is described.