Calculating Dynamic CMRO2 Changes Using CBF & BOLD fMRI

描述（由申请人提供）：神经元活动产生的脑代谢耗氧量（CMR02）的变化与脑组织功能的能量学直接相关。这些变化促成了血氧水平依赖（BOLD）功能磁共振成像信号，并部分负责在脑功能的多学科研究中广泛使用BOLD功能磁共振成像。从BOLD fMRI数据中确定CMR02反应的大小和动态不仅对揭示BOLD反应的基础，而且对理解脑功能具有很大的意义。本提案的目的是利用fMRI数据确定神经激活引起的CMR02的动态变化，并研究瞬时氧运输和消耗的假设是否有效。为此，无创和有创测量CBF、脑血容量（CBV）、组织氧张力（PO2tiss）。利用MRI（9.4特斯拉扫描仪）和非MRI方法，在一个成熟的大鼠躯体感觉模型中获得固有信号（OIS）和BOLD测量的光学成像。作为这个项目的结果，一种利用fMRI数据来确定CMR02动态变化的方法将被开发和验证。这些方法可用于临床环境，以评估组织活力和功能在大脑以及其他器官。

DESCRIPTION (provided by applicant): Changes in the cerebral metabolic rate of oxygen consumption (CMR02) produced by neuronal activity are directly related to the energetics of brain tissue function. These changes contribute to the blood oxygenation level dependent (BOLD) fMRI signal and have been partly responsible for the extensive use of BOLD fMRI in multi-disciplinary studies of brain function. Determining the magnitude and dynamics of CMR02 responses from BOLD fMRI data has been of great interest not only to unravel the underpinning of the BOLD response, but also in the understanding of brain function. The goals of this proposal are to determine the dynamic changes in CMR02 elicited by neural activation using fMRI data and investigate whether the assumption of instantaneous oxygen transport and consumption is valid for this purpose. To this end, non-invasive and invasive measurements of CBF, cerebral blood volume (CBV), tissue oxygen tension (PO2tiss). optical imaging of intrinsic signals (OIS) and BOLD measurements will be acquired using MRI (9.4 Tesla scanner) and non-MRI methodologies in a well-established rat somato-sensory model. As a result of this proposed project, a method to determine the dynamic changes in CMR02 using fMRI data will be developed and validated. These methods can be used in the clinical setting to assess tissue viability and function in the brain as well as other organs.