Retinal Ischemia Treatment by Oxygen Nanobubbles

摘要 视网膜组织由于其高代谢而对氧合不足高度敏感。 活动事实上，人们普遍认为视网膜缺氧在这些事件中起着核心作用， 导致神经元细胞损伤和由于局部缺血引起的死亡，并与视觉损伤有关 由于视网膜血管阻塞和糖尿病视网膜病变。以前的研究调查了 使用高压氧治疗视网膜缺血。然而，这种有效性 向内层视网膜提供足够氧气的治疗是有限的 不存在.我们解决了开发治疗干预措施的未满足需求， 保护视网膜组织，防止缺血性损伤导致的视力丧失。我们建议 利用氧纳米泡（ONB）对视网膜内层进行氧合的概念， 氧作为视网膜缺血的新的治疗方式。我们 目的是：开发一种用于合成ONB的一致配方，并评估其 物理和生化特性;确定ONB给药的最佳时间 改善缺血诱导的视网膜氧度量和视觉损害的治疗 功能;并根据一系列综合指标确定ONBs治疗的疗效。 结果，即视网膜氧指标、视功能、视网膜厚度和生化指标 途径。我们希望我们的研究结果将作为一个创新的强有力的初步证据。 治疗干预以减轻缺血性损伤，保护视网膜组织免受损伤，以及 防止视力丧失。

Abstract The retinal tissue is highly susceptible to inadequate oxygenation due to its high metabolic activity. Indeed, it is commonly accepted that retinal hypoxia plays a central role in the events leading to neuronal cell damage and death due to ischemia and implicated in visual impairments due to retinal vascular occlusions and diabetic retinopathy. Previous studies have investigated the use of hyperbaric oxygen treatment for retinal ischemia. However, the effectiveness of this treatment to supply adequate oxygen to the inner retina is limited and a standard of care does not exist. We address an unmet need for the development of therapeutic interventions to oxygenate the retinal tissue and prevent vision loss from an ischemic insult. We propose the concept of oxygenation of the inner retina with oxygen nanobubbles (ONBs) that can release oxygen over an extended period of time as a novel treatment modality for retinal ischemia. Our aims are to: develop a consistent formulation for the synthesis of ONBs and evaluate its physical and biochemical properties; identify the optimum time for administration of ONBs treatment to improve ischemia-induced impairments of retinal oxygen metrics and visual function; and determine the efficacy of ONBs treatment based on a series of comprehensive outcomes, namely, retinal oxygen metrics, visual function, retinal thickness, and biochemical pathways. We expect our findings will serve as strong preliminary evidence of an innovative therapeutic intervention to mitigate ischemic insult, preserve the retinal tissue from injury, and prevent vision loss.