Lab Automation for a Genomics Shared Resources

项目摘要/摘要 此应用程序的目标是改进和简化 分析和翻译基因组学(ATG)共享资源，专注于下一代的关键核心设施 新墨西哥大学综合癌症中心的测序和基因组学技术 (联合国苏丹特派团)。我们正在为核酸提取设备申请资金。具体地说，一辆起亚EZ2 连接全自动核酸提纯机器人。将此仪器添加到ATG共享资源将 对已经到位并将提高吞吐量的成功工作流进行现代化和自动化， 扩展服务范围，为用户降低成本，加快周转速度。所请求的 设备将使现在由ATG技术人员手工执行的程序自动化，使他们能够 专注于更复杂的下游活动，如准备各种类型的测序库。这个 所要求的设备不是获取实验数据的科学研究仪器，但它将 显著增强支持操作，并将使用户社区和多个研究项目受益 许多调查人员。ATG得到了CCC特派团的部分支持，该特派团是NCI指定的仅有的三个特派团之一 美国国立卫生研究院机构发展奖(IDEA)计划中的综合癌症中心-符合条件的州。 北卡罗来纳大学的实验室广泛使用ATG。在过去的5年里，ATG向73个UNM实验室开出了近 基因组学服务的175万美元使用费。所要求的设备将补充和增强 最近对新的测序仪器的投资，由联合国儿童基金会和联合国监测和监测健康中心提供资金 并将极大地惠及广泛的用户，包括联东综合团成员和非成员， 他们利用ATG提供的基因组学服务。

Project Summary/Abstract The goal of this application is to improve and streamline the operating processes and procedures in the Analytical and Translational Genomics (ATG) Shared Resource, a key core facility focused on next-generation sequencing and genomics technologies in the University of New Mexico Comprehensive Cancer Center (UNMCCC). We are requesting funds for nucleic acid extraction equipment. Specifically, a Qiagen EZ2 Connect automated nucleic acid purification robot. Adding this instrument to the ATG Shared Resource will modernize and automate the successful workflows that are already in place and will improve throughput, expand the menu of services and lead to reduced costs and faster turnaround for our users. The requested equipment will automate procedures that are now performed by hand by the ATG technical staff, allowing them to focus on more complex downstream activities, such as preparing various types of sequencing libraries. The requested equipment is not a scientific research instrument that acquires experimental data, but it will significantly enhance support operations and will benefit the user community and multiple research projects of many investigators. ATG is partially supported by UNMCCC, one of only three NCI-Designated Comprehensive Cancer Centers in an NIH Institutional Development Award (IDeA) Program-eligible state. ATG is widely used by laboratories at UNM. In the last 5 years ATG billed 73 UNM laboratories for nearly $1.75 million in user fees for genomics services. The requested equipment will complement and augment recent investments in new sequencing instruments, made with funds from UNMCCC and the UNM Health Sciences Center, and will greatly benefit a wide range of users, both UNMCCC members and non-members, who utilize the genomics services provided by ATG.