DEFINING THE HCV INFECTIOUS WINDOW PERIOD IN THE CHIMPANZEE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 丙型肝炎病毒(丙型肝炎病毒)的感染窗口期，即接触病毒和首次检测到病毒RNA之间的时间，没有明确的定义。通过设立窗口期，可以进一步降低和/或预防血液制品传播丙型肝炎病毒的风险。目的是在黑猩猩模型中定义和描述丙型肝炎病毒的感染窗口期。已经选择了两只黑猩猩，并测定了基线肝酶水平。丙型肝炎病毒临界阳性和阴性血浆面板正被放在一起进行拟议的研究。其中一只黑猩猩被静脉注射来自核酸检测(NAT)阴性的五名个体捐赠者中的每一名捐赠者的50毫升血浆。动物被感染，表明丙型肝炎病毒的存在低于NAT的检测水平。目前正在进行其他病毒学和免疫学检测。病毒学分析表明，该动物迅速清除了感染，没有血清转换。第二只动物的接种计划被搁置，直到最终决定是否使用不同的血浆面板。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The infectious window period for hepatitis C virus (HCV), the time between exposure to the virus and the first detection of viral RNA, is not well defined. By establishing the window period, the risk of transmission of HCV by blood products can be further reduced and or prevented. The objective is to define and characterize the infectious window period for HCV in the chimpanzee model. Two chimpanzees have been selected and baseline liver enzyme levels have been determined. HCV borderline positive and negative plasma panels are being put together for the proposed studies. One chimpanzee was IV infused with 50 ml of plasma from each of five individual donors from a panel negative for HCV by nucleic acid testing (NAT). The animals become infected indicating that HCV was present below the level of detection of NAT. Additional virological and immunological assays are being performed at the represent time. Virological assays indicated that the animal rapidly cleared the infection and did not seroconvert. The planned inoculation of the second animal is on hold until a final decision is made on using a different plasma panel.