Translational Methods/Facilities Core

翻译方法/设施（Translational Methods/Facilities）核心将提供最先进的遗传分析以及 小鼠模型的开发，表征和评估的所有组成部分的联盟， 神经精神表型学。三个不同但相互作用的单位在业务上确定了 TMF-核心： 遗传研究股将负责生成和分析所有遗传数据， 联合会将进行多项关联研究。 BAG转基因单位将使用BAG转基因或通过杂交创建新的小鼠模型 a）通过遗传学方法测试基因型/表型关联的细胞病因学， 拯救策略，B）驱动荧光蛋白的细胞特异性表达，以鉴定 可以通过突变基因扰动，以及c）对所鉴定的候选基因的贡献进行建模 和/或从正在进行的遗传关联分析中预期显著改变记忆 过程或反应抑制。 啮齿动物表型分析单位与人类翻译应用核心密切合作 将设计，验证和进行行为测定表型小鼠模型所使用的 财团 这三个单位利用加州大学洛杉矶分校相当多的物质和智力资源。这种杠杆作用将 使TMF-Core能够经济有效地提供信息遗传/表型数据库， 在整个联盟中共享动物模型和有效的行为评估， 加州大学洛杉矶分校以外的研究团体。

The Translational Methods/Facilities (TMF) Core will provide state of the art genetic analysis as well as mouse model development, characterization and assessment for all components of the Consortium for Neuropsychiatric Phenomics. Three distinct but interactive units operationally define the specific aims of the TMF-Core: ¿ The Genetic Studies Unit will be responsible for generating and analyzing all genetic data in the multiple association studies to be conducted by the Consortium. ¿ The BAG Transgenic Unit will create novel murine models using BAG transgenes or by crossing established mutant lines to a) test the cellular etiology of genotype/phenotype associations by genetic rescue strategies, b) drive cell-specific expression of fluorescent proteins to identify CNS circuitry that may be perturbed via mutant genes and c) model the contribution of candidate genes identified and/or anticipated from ongoing genetic association analysis to significantly modify memory processes or response inhibition. ¿ The Rodent Phenotyping Unit in close collaboration with the Human Translational Applications Core will design, validate and conduct behavioral assays to phenotype mouse models used by the Consortium. The three units leverage considerable physical and intellectual resources at UCLA. Such leveraging will enable the TMF-Core to cost-effectively and efficiently provide informational genetic/phenotype databases, shared animal models and validated behavioral assessments throughout the Consortium and subsequently to the research community outside of UCLA.