ISLET PROCUREMENT AND ANALYSIS CORE

小岛采购和分析核心是范德比尔特DRTC的一个新核心。小岛生物学、发育、 和功能是DRTC的主要研究领域，研究人员研究各种与胰岛相关的过程 范围从细胞内信号，1型糖尿病的免疫学，胰岛丰富的转录因子，到 胰岛移植。这些DRTC附属调查人员的许多实验范例的核心是 进入啮齿动物、猪或人的胰岛。这个核心的目的是为了促进研究 通过提供高质量、特征良好的胰岛并协助 使用这些胰岛的研究中的研究人员。范德比尔特的一个小鼠胰岛隔离核心已经投入使用 在范德比尔特老鼠代谢表型中心(MMPC)的赞助下，但它的设计就是这样的 它不适合处理由于DRTC的数量和活动而需要的胰岛体积 范德比尔特内部的调查人员继续增长。此外，范德比尔特大学的一些研究人员正在研究 与小鼠胰岛发育和功能有关的机制，但限制进入人类胰岛的速度减慢 将他们的观察转化为人类小岛的竞技场。因此，在此应用程序中，我们建议创建 范德比尔特小岛采购和分析核心，将由NIDDK资助的联合支助 MMPC和DRTC，并将大幅增加DRTC附属调查人员访问 胰岛。该中心提供的服务包括：1)分离啮齿动物胰岛；2)纯化 和人类胰岛的处理(从NIH/JDRF人类胰岛分配方案收到)；以及3) 通过评估细胞灌流中的胰岛素分泌来表征所有物种的胰岛制剂 系统。这一核心将扩大DRTC附属调查人员进行糖尿病相关研究的能力 并将与其他DRTC支持的核心交互。例如，可以在细胞中研究孤立的胰岛 成像共享资源，分泌的胰岛激素将在激素分析核心中进行检测，或 评估体内胰岛素分泌的平行研究可在《代谢生理学共享》杂志上进行 资源，并与孤立胰岛的体外研究相关联。这一核心将提供基本服务， 在下一个资金周期中支持DRTC附属调查人员的研究。

The Islet Procurement and Analysis Core is a new core of the Vanderbilt DRTC. Islet biology, development, and function are major DRTC research areas with investigators studying a variety of islet-related processes ranging from intracellular signaling, the immunology of type 1 diabetes, islet-enriched transcription factors, to islet transplantation. Central to many experimental paradigms of these DRTC-affiliated investigators is access to rodent, porcine, or human pancreatic islets. The objective of this core is to facilitate the research of DRTC-affiliated investigators by providing high quality, well-characterized pancreatic islets and to assist investigators in studies that use these islets. A mouse islet isolation core at Vanderbilt has been operational under the auspices of the Vanderbilt Mouse Metabolic Phenotyping Center (MMPC), but its design is such that it is not well-suited for handling the volume of islets needed as the number and activity of DRTC investigators within Vanderbilt continue to grow. In addition, a number of Vanderbilt researchers are studying the mechanisms involved in mouse islet development and function, but limited access to human islets slows translation of their observations into the human islet arena. Thus, in this application, we propose to create the Vanderbilt Islet Procurement and Analysis Core, which will be jointly supported by the NIDDK-funded MMPC and the DRTC and will substantially increase the access of DRTC-affiliated investigators to pancreatic islets. The services offered by this core are: 1) isolation of rodent pancreatic islets; 2) purification and handling of human islets (received from NIH/JDRF human islet distribution program); and 3) characterization of islet preparations from all species by assessment of insulin secretion in a cell perifusion system. This core will expand the ability of DRTC-affiliated investigators to perform diabetes-related research and will interact with other DRTC-supported cores. For example, isolated islets may be studied in the Cell Imaging Shared Resource, secreted islet hormones would be assayed in the Hormone Assay Core, or parallel studies assessing in vivo insulin secretion can be performed in the Metabolic Physiology Shared Resource and correlated with in vitro studies in isolated islets. This core will provide essential services that support the research of DRTC-affiliated investigators in the next funding cycle.