NONINVASIVE ASSESSMENT OF INSULIN RESISTANCE: PHASE I

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 背景：胰岛素抵抗是许多慢性疾病发病机制的标志，并已成为一个主要的健康问题。 找到一种既易于管理又准确的替代非侵入性测试可能会导致胰岛素抵抗的早期检测，从而使个人能够寻求更早的成本预防性治疗方案。 具体目标和程序：长期目标是开发胰岛素抵抗的非侵入性测试。 实验设计：每例受试者将有一个筛选期，并根据这些结果要求返回医院住院，届时将完成OGTT和呼吸测试。 所有入院的受试者将接受外周静脉采血。 通过在2个基线血液样品后给予受试者75克Glucola来施用OGTT。 然后测试持续10小时，在选定的时间点采血。 呼吸样本将在与OGTT相同的时间点采集。 将要求受试者进行完整的呼吸，然后通过吸管将整个呼吸呼出到试管中。在完全呼气之前，拔出吸管，并将螺旋盖放回试管上。 重要性：这项研究的成功结果将为制造一种产品提供科学依据，该产品将有助于通过使用非侵入性技术准确检测胰岛素抵抗。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: Insulin resistance is the hallmark of pathogenesis of a number of chronic disorders and have become a major health concern. Finding an alternative non-invasive test that is both easy to administer and accurate may lead to earlier detection of insulin resistance, thus enabling individuals to seek earlier cost preventative treatment options. Specific Aims and Procedures: The long term objective to develop a non-invasive test of insulin resistance. Experimental Design: Each subject will have a screening period and based on those results will be asked to return for a hospital admission at which time the OGTT and the breath test will be completed. All admitted subjects will receive a peripherial IV for blood sampling. The OGTT is administered by giving the subject 75 grams of Glucola following 2 baseline blood samples. The test then continues for 10 hours with blood sampling at selected time points. The breath samples will be collected at the same time points as the OGTT. The subject will be asked to take a full breath and then the entire breath is exhaled through a straw into a tube. Before exhaling completely, the straw is withdrawn and the screw cap is replaced on the tube. Significance: A successful outcome of this research will lead to the scientific basis for manufacturing a product that will help to accurately detect insulin resistance by using a noninvasive technique.