NONINVASIVE ASSESSMENT OF INSULIN RESISTANCE: PHASE I

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。背景：胰岛素抵抗是许多慢性疾病发病机制的标志，已成为一个主要的健康问题。寻找一种易于实施且准确的非侵入性检测方法，可能会导致更早发现胰岛素抵抗，从而使个人能够寻求更早的预防性治疗方案。具体目的和程序：长期目标是发展胰岛素抵抗的非侵入性测试。实验设计：每个受试者将有一个筛选期，根据这些结果将被要求返回医院住院，届时将完成OGTT和呼吸测试。所有住院患者将接受外周静脉抽血。OGTT的方法是给受试者注射75克葡糖苷葡萄糖，然后采集两份基线血液样本。然后，在选定的时间点继续进行10小时的血液采样。呼气样本将在OGTT的同一时间点采集。受试者将被要求进行一次完整的呼吸，然后将整个呼吸通过吸管呼到一个管子中。在完全呼气之前，取出吸管，并更换管上的螺旋盖。意义：这项研究的成功结果将为制造一种产品提供科学基础，该产品将有助于通过使用无创技术准确检测胰岛素抵抗。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: Insulin resistance is the hallmark of pathogenesis of a number of chronic disorders and have become a major health concern. Finding an alternative non-invasive test that is both easy to administer and accurate may lead to earlier detection of insulin resistance, thus enabling individuals to seek earlier cost preventative treatment options. Specific Aims and Procedures: The long term objective to develop a non-invasive test of insulin resistance. Experimental Design: Each subject will have a screening period and based on those results will be asked to return for a hospital admission at which time the OGTT and the breath test will be completed. All admitted subjects will receive a peripherial IV for blood sampling. The OGTT is administered by giving the subject 75 grams of Glucola following 2 baseline blood samples. The test then continues for 10 hours with blood sampling at selected time points. The breath samples will be collected at the same time points as the OGTT. The subject will be asked to take a full breath and then the entire breath is exhaled through a straw into a tube. Before exhaling completely, the straw is withdrawn and the screw cap is replaced on the tube. Significance: A successful outcome of this research will lead to the scientific basis for manufacturing a product that will help to accurately detect insulin resistance by using a noninvasive technique.