A PHASE I TRIAL EXAMINING SKELETAL MUSCLE PERFUSION AND MUSCLE PROTEIN

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。背景：这项研究的目的是确定肌肉血流对肌肉组织利用肌肉蛋白质(即氨基酸)的积木以产生更多肌肉的速度的影响。这将帮助我们更好地了解衰老引起的血液流动变化将如何影响肌肉对营养的反应。假设：确定补充氨基酸是否会改善有氧运动或不进行有氧运动的年轻人和老年人的肌肉血流量。具体目标和程序：我们的目标是通过比较和测量健康的年轻和老年志愿者的肌肉组织内的血流量，找到预防与年龄相关的肌肉损失的方法。血流量的测量将使用各种技术：1)使用一种名为硝普钠(SNP)的药物，它可以扩张你大腿的血管；2)使用ICG(吲哚青绿)技术；3)使用微泡和超声波技术；4)使用应变计体积描记仪。实验设计：每个受试者将参加两个9小时的输液研究。研究将每隔一个月进行一次。每个受试者将被随机分配参加实验1、2或3。意义：这些研究的结果可以提供大量与随着年龄增长而逐渐失去肌肉质量和功能有关的信息。此外，这些数据可能会提出应对和防止与年龄相关的变化的策略。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Brief Background: The purpose of this study is to determine the influence of muscle blood flow on the rate at which the building blocks of muscle protein (i.e. amino acids) are used by the muscle tissue to produce more muscle. This will help us better understand how changes in blood flow caused by aging will affect how muscle responds to nutrition. Hypothesis: To determine whether or not amino acid supplementation will improve blood flow to the muscle in both young and elderly subjects with or without aerobic exercise. Specific Aims and Procedures: Our aim is to find ways of preventing age-related muscle loss by comparing and measuring blood flow within the muscle tissue of healthy young and elderly volunteers. Blood flow will be measured using various techniques: 1) with the use of a medicine, called Sodium Nitroprusside (SNP), that dilates the vessels in your thigh, 2) with the use of the ICG (indocyanine green) technique, 3) with the use of micro bubbles and the ultrasound technique, 4) with the use of strain gauge plethysmography. Experimental Design: Each subject will participate in two 9-hour infusion studies. The studies will take place one month apart. Each subject will be randomly assigned to participate in either Experiment #1, 2 or 3. Significance: The results from these studies could provide a wealth of information related to the progressive loss of muscle mass and function that accompanies advancing age. Further, the data may suggest strategies to combat and prevent age-related changes.