EXTREME DIETARY CARBOHYDRATE RESTRICTION EFFECT ON HEPATIC GLUCOSE PRODUCTION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 肥胖及其伴发疾病是21世纪医疗行业面临的最普遍和最具挑战性的疾病之一。从历史上看，美国国立卫生研究院目前的减肥饮食建议很难启动和维持。此外，为糖尿病患者推荐的饮食在短期内被证明是有效的，但在长期内不能帮助控制血糖。新流行的碳水化合物限制饮食可能会为那些寻求减肥和改善血糖控制的人提供比低脂肪、高碳水化合物、卡路里限制饮食更大的好处。然而，人们对这两种饮食对肝脏葡萄糖代谢的影响知之甚少。 本研究旨在从肝脏产生葡萄糖的前体(糖原、甘油和丙酮酸)的来源以及通过Krebs循环、磷酸烯醇式丙酮酸后羧酸激酶和丙酮酸循环的中间产物流量方面，确定严重的碳水化合物限制对肝脏葡萄糖代谢的影响。确定肝脏葡萄糖代谢的变化将有助于理解限制碳水化合物饮食可能具有的好处，并可能有助于确定哪些人更有可能受益于饮食碳水化合物限制而不是脂肪和卡路里限制(即，制定饮食计划的能力，而不是目前的一刀切方法)。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Obesity and its associatd co-morbidities are among the most prevalent and challengin conditions confronting the medical profession in the 21st century. current dietary recommendations from the NIH for weight loss have historically proven difficult to initiate and maintain. Additionally, recommended diets for diabetic patients have proven effective in the short-term but fall short in aiding glycemic control in the long-term. The newly popular carbohydrate-restricted diet may offer benefits to those seeking weight reduction and improved glycemic control greater than those realized with a low-fat, high-carbohydrate, calorie-restricted diet. However, little is known about the effect of either of these diets on hepatic gluose metabolism. This study seeks to determine the impact of severe dietary carbohydrate restriction on hepatic glucose metabolism in terms of the sources of precursors for glucose produced by the liver (glycogen, glycerol, and pyruvate) and the flux of intermediates through the Krebs' cycle, postphoenolpyruvate carboxykinase, and pyruvate recycling. Identifying alterations in hepatic glucose metabolism will aid in the understanding of the benefits a carbohydrate-restricted diet may have and may aid in identification of those persons more likely to benefit from dietary carbohydrate restriction versus fat and calorie restriction (i.e., the ability to tailor a diet plan as opposed to the current one-sixe-fits-all approach).