NE COBRE: MICROSCOPY CORE RESEARCH FACILITY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 显微镜核心研究设施(MCRF)于1999年成为生物技术中心(UNL)的多用户设施，现在是内布拉斯加州利用最频繁的核心之一。MCRF为来自学术和工业组织的所有研究人员提供最先进的仪器和专业服务。显微镜设施配备了以下主要成像系统和仪器。三台新的日立电子显微镜：H7500-I型电子显微镜、S-4700场发射扫描电子显微镜和S-3000N变压扫描电子显微镜。两套奥林巴斯共聚焦激光扫描显微镜系统，可同时显示双荧光或三荧光标记材料。配备高灵敏度视频/数码相机的两台先进荧光显微镜：奥林巴斯AX70立式荧光显微镜和尼康TE-300倒置荧光显微镜。尼康SMZ-800荧光立体显微镜。一台徕卡CM-1900冷冻切片机，用于快速制备冰冻切片和超显微切片。MCRF经理和工作人员与164个教职员工中的至少30个(仅来自UNL的24个系)建立了强大的研究合作关系，每年有60-80个教职员工团体使用。该中心还参与了本科生和研究生教育/培训计划，并在过去一年为大约300名学生组织了成像系统的演示。此外，MCRF一直积极参与赠款申请(如NCV、Redox Center和Cell Signals，以及许多个人调查人员)，并一直是NCV的关键组成部分。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Microscopy Core Research Facility (MCRF) became a multi-user facility of the Center of Biotechnology (UNL) in 1999 and is now one of the most heavily utilized cores in Nebraska. The MCRF provides the-state-of-art instrumentation and professional services to all researchers from both academic and industrial organizations. The Microscopy Facility is equipped with the following major imaging systems and instruments. Three new Hitachi electron microscopes: H7500-I TEM, S-4700 Field-emission SEM, and S-3000N variable pressure SEM. Two Olympus confocal laser scanning microscope systems with the capacity for simultaneous display of double or triple fluorescence labeled materials. Two advanced fluorescence microscopes with high-sensitivity video/digital cameras: Olympus AX70 upright fluorescence microscope and Nikon TE-300 inverted fluorescence microscope. A Nikon SMZ-800 fluorescence stereo microscope. A Leica CM-1900 cryostat microtome for rapid preparation of frozen sections and Ultra-microtomes. The MCRF Manager and staff have set up strong research collaborations with many at least 30 of the 164 faculty/PIs (from 24 departments in UNL alone) and has been utilized by 60-80 faculty groups annually. The Core has also been involved in undergraduate and graduate education/training programs and has organized demonstrations of imaging systems for ~ 300 students during the past year. In addition, the MCRF has been actively participating in grant application (such as NCV, Redox Center, and Cell Signaling, as well as many individual investigators) and has been a key component of the NCV.