CORE C: HISTOPATHOLOGY CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该中心为COBRE和其他MMCRI研究者提供组织学服务。 这包括冷冻、石蜡或塑料包埋标本的组织固定、包埋和切片。组织学染色剂包括苏木精-伊红、三色、von Kossa、地衣红、货车Gieson（以及其他需要的染色剂）。还提供了用多种抗体的免疫组织化学染色。核心是由一个全职的组织学家超过25年的经验，在该领域（凯瑟琳承运人）。核心主管（Rehhard Lindner）负责监督核心操作，并协助解释和评价组织学标本。 核心使用以下设备：用于冷冻切片的Microm HM 505 E低温恒温器、用于石蜡和塑料切片的Microm HM 355 S和Microm HM 325切片机、Fisher HistoCenter包埋站、用于石蜡渗透的Lab-Line真空烘箱型号3618和VWR真空烘箱型号1410、载玻片加热器和水浴。对于集中组织学操作，通过COBRE奖提供的资金，自动组织处理器（Leica TP 1020）上线。2005年，一台自动载玻片染色机（Leica Autostainer XL）和盖片站（Hyperclean）上线。 Kathleen Carrier执行所有日常操作，包括组织处理（Leica TP 1020）和特殊染色;免疫组织化学;申请表;订购和维护记录;选择方案开发;和共聚焦显微镜。此外，在线还有HM 505 E低温恒温器、HM 355 S和HM 325切片机、HistoCenter包埋站和用于石蜡渗透的烤箱。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This Core provides histology services to COBRE and other MMCRI investigators. This includes tissue fixation, embedding, and sectioning of frozen, paraffin or plastic embedded specimens. Histological stains include Hematoxylin-Eosin, Trichrome, von Kossa, Orcein, van Gieson (and others as needed). Immunohistochemical staining with a variety of antibodies is also provided. The Core is operated by a full time histologist with more than 25 years of experience in the field (Kathleen Carrier). The Core director (Volkhard Lindner) oversees Core operations and assists in the interpretation and evaluation of histology specimens. The Core uses the following equipment: Microm HM505E Cryostat for frozen sections, A Microm HM355S and Microm HM325 microtome for paraffin and plastic sections, A Fisher HistoCenter embedding station, Lab-Line Vacuum oven Model 3618 and VWR vacuum oven model 1410 for paraffin infiltration, Slide Warmers and water baths. For the centralized histology operation an automated tissue processor (Leica TP1020) was brought on line through funds provided by this COBRE award. An automated slide stainer (Leica Autostainer XL) and cover-slipping station (Hyperclean) were brought online in 2005. Kathleen Carrier performs all day-to-day operations including tissue processing (Leica TP1020) & special stains; immunohistochemistry; request forms; ordering and maintenance records; select protocol development; and confocal microscopy. Also, on-line are a HM505E Cryostat, HM355S and HM325 microtomes, HistoCenter embedding station, and ovens for paraffin infiltration.