LSU VETERINARY COBRE: MOLECULAR BIOLOGY AND IMMUNOLOGY CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 路易斯安那州立大学COBRE核心实验室（LSU-MBIC）在运营上位于路易斯安那州立大学兽医学院生物技术和分子医学部（BIOMMED）的现有集中设施内。LSU-MBIC将其支持功能集中在分子生物学/重组DNA服务、生物信息学和小鼠免疫参数的分子评价上。分子生物学服务和试剂包括标准服务，如DNA测序、实时PCR运行、重组质粒构建和纯化以及cDNA文库构建。先进的服务包括使用与LSU彭宁顿生物医学研究中心（PBRC-BU）的生物信息学单位一起提供的先进生物信息学能力处理和分析微阵列实验。此外，LSU-MBIC还提供了小鼠细胞因子的实时PCR分析，荧光激活分选（FACS）分析，以及与兽医学院合作的共聚焦显微镜集中设施，致力于FACS和光学显微镜。LSU-MBIC的具体目标是：（1）为COBRE研究人员提供先进的分子生物学知识，试剂和服务，包括生物信息学。(2)促进使用先进的分子免疫测定法来评估小鼠的免疫反应，以及使用路易斯安那州立大学兽医学院的其他研究资源。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Louisiana State University COBRE Core laboratory (LSU-MBIC) is operationally housed within the existing centralized facilities of the Division of Biotechnology and Molecular Medicine (BIOMMED) at the LSU School of Veterinary Medicine. The LSU-MBIC has concentrated its support functions on molecular biology/recombinant DNA services, bioinformatics and molecular evaluation of immunological parameters in mice. Molecular biological services and reagents have included standard services such as DNA sequencing, real-time PCR runs, recombinant plasmid construction and purification, and cDNA library construction. Advanced services have included processing and analysis of microarray experiments using advanced bioinformatics capabilities offered in conjunction with the Bioinformatics Unit of the LSU Pennington Biomedical Research Center (PBRC-BU). In addition, LSU-MBIC has offered real-time PCR analysis of mouse cytokines, Fluorescent Activated Sorting (FACS) analysis, and confocal microscopy in collaboration with the School of Veterinary Medicine Centralized Facilities dedicated to FACS and Light Microscopy. The specific aims of the LSU-MBIC are: (1) To provide advanced molecular biological know-how, reagents and services including bioinformatics to COBRE investigators. (2) To facilitate the use of advanced molecular immunoassays for the evaluation of immune responses of mice, and the use of other research resources of the LSU School of Veterinary Medicine.