UCLA NINDS/NIMH Microarray Center

描述(由申请人提供)：此竞争性补充请求是为了升级Solexa/llLumina 1G Sequencer的光学系统和基本调用算法，以便1)证明每个Sequencer的容量可以很容易地增强，以及2)极大地增加在联盟内使用这一重要新技术的神经科学研究人员的能力。对这项技术的需求很大(需要1600多个样本容量)，但财团内部的能力有限。这项提议寻求建立在加州大学洛杉矶分校大规模并行测序的既定经验和我们在扫描仪制造方面的经验，以增强加州大学洛杉矶分校的能力，并使该财团在神经科学家接受基于测序的基因组分析方法时进一步扩大服务。目标1：构建并测试1G Sequencer的成像组件，它在对Illumina 1G Sequencer的菌落进行成像时速度快16倍。目标2：在商用Illumina 1G测序仪中实现新的成像系统。目标3：将基于Solexa/llLumina的测序服务扩展到NIH神经科学微阵列中心的用户。

DESCRIPTION (provided by applicant): This competing supplement request is to upgrade the optical system and base calling algorithms of a Solexa/lllumina 1G Sequencer in order to 1 )demonstrate that the capacity of each sequencer can be readily augmented, and 2) to greatly increase capacity for neuroscience investigators using this important new technology within the Consortium. There is a large demand for this technology (over 1600 sample capacity desired), yet only a limited capacity within the consortium. This proposal seeks to build upon the established experience at the UCLA site with massively parallel sequencing and our experience in scanner fabrication to augment capacity at the UCLA site and poise the consortium to further expand services as neuroscientists embrace sequencing based approaches to genomic analysis. Aim 1: To build and test an imaging component for the 1G Sequencer which is 16 fold faster at imaging colonies from the Illumina 1G Sequencer. Aim 2: Implement new imaging system within commercial Illumina 1G Sequencer. Aim 3: Expand Solexa/lllumina based sequencing services to NIH Neuroscience Microarray Center users.