RADIATION DAMAGE AS A FUNCTION OF WAVELENGTH

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 为了在双波长MAD实验中微调数据收集参数，我们还试图确定辐射损伤如何影响吸收边上不同能量的数据。 为此，我们从用Galeodinium衍生的不同溶菌酶晶体收集数据。 在四个不同的点收集连续的数据集：在吸收边缘的低能量侧，拐点，吸收峰和边缘的高能量侧。 我们检查了半胱氨酸、硫还原和威尔逊温度因子作为辐射损伤的指标。 很难从数据中得出结论，在不同的能量下是否观察到不同数量的特定损伤;温度因子在较低的能量下增加，这表明晶体上的剂量不是影响晶体所遭受的损伤量的唯一因素。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In order to fine-tune data collection parameters in two-wavelength MAD experiments, we also seeked to determine how radiation damage affects the data at different energies over the absorption edge. To do this we collected data from different lysozyme crystals derivatized with Galeodinium. Successive data sets were collected at four different points: on the low energy side of absorption edge, inflection point, absorption peak and on the high energy side of the edge. We examined cysteine Sulphur reduction and the Wilson temperature factor as the indicators of radiation damage. It was difficult to conclude from the data whether a different amount of specific damage was observed at different energies; The temperature factor increases at lower energies, which suggests that the dose on the crystal is not the only factor affecting the amount of damage suffered by the crystals.