TUNABLE X-RAY CANCER PHOTOTHERAPY

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们希望测试的假设，即单色X射线辐射的K-边缘和以上的各种重金属结合到基板内的肿瘤细胞是更有效的，传统的放射治疗杀死肿瘤细胞在体外。 该计划是SLAC速调管部门康普顿光源（CLS）项目的一部分，旨在开发一种可调的紧凑型准单能X射线源，用于癌症检测和治疗。 虽然CLS正在开发中，我们建议使用光束线在斯坦福大学同步辐射实验室（SSRL）的研究剂量增强X射线光疗。 拟议的工作将使用SSRL光束线照射已建立的高Z原子标记的细胞系。 将通过传统菌落计数和生物发光试验生成的剂量-反应曲线评估剂量增强。 放射增敏剂的候选物包括碘、钆、铟、铂和金。 样品将在这些元素的K边缘以上0-30 keV的能量下辐照。 尽管SSRL光束线在这些能量下产生相对低的通量，但细胞培养几何形状可以调整为小光束尺寸，并且样品可以被照射长时间（小时）以达到更高的积分光子强度。 在这些实验中，允许更宽的带宽（高达1千电子伏）作为达到更高通量的权衡。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We wish to test the hypothesis that monochromatic x-ray radiation at and above the K-edge of various heavy metals bonded to substrates within neoplastic cells is more effective that conventional radiation therapy for killing neoplastic cells in vitro. This program is part of the Compton Light Source (CLS)project at the SLAC Klystron Department to develop a tunable, compact source of quasi-monoenergetic x-rays for use in cancer detection and treatment. While the CLS is under development, we propose to use a beamline at the Stanford Synchrotron Radiation Laboratory (SSRL) for studying dose enhancement in x-ray phototherapy. The proposed work will use an SSRL beamline to irradiate established cell lines labeled with high-Z atoms. Dose enhancement will be assessed through dose-response curves generated both by traditional colony counting and by bioluminescence assay. Candidates for radiosensitizing agents include Iodine, Gadolinium, Indium, Platinum, and Gold. Saples will be irradiated at energies 0-30 keV above the K-edges of these elements. Although SSRL beamlines produce relatively low fluxes at these energies, cell culture geometry can be adjusted to the small beam size, and samples may be irradiated for long times (hours) to reach higher integrated photon intensities. Broader bandwidths (up to 1 keV) are permitted in these experiments as a trade-off for reaching higher fluxes.