STUDIES OF METALLOPROTEINS (PFL ACTIVATING ENZYME, HPPE, NIKR, SYRB2)

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 金属和金属蛋白是许多细胞过程所必需的。 本实验室利用大分子晶体学来研究金属蛋白的结构和功能。 这些蛋白质包括镍调节蛋白NikR，一种DNA结合蛋白;丙酮酸-甲酸裂解酶激活酶（PFL-AE），一种通过在丙酮酸甲酸裂解酶上形成蛋白质结合的甘氨酰基自由基而参与厌氧代谢的自由基SAM酶;羟丙基膦酸环氧酶（HppE），其使用单核铁位点催化独特的环氧化;和SyrB 2，一种参与天然产物生物合成的单核铁蛋白。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Metals and metalloproteins are essential for many cellular processes. Our laboratory uses macromolecular crystallography to study the structure and function of metalloproteins. These proteins include nickel regulatory protein NikR, a DNA-binding protein; pyruvate-formate lyase activating enzyme (PFL-AE), a Radical SAM enzyme involved in anaerobic metabolism by formation of a protein bound glycyl radical on pyruvate formate lyase; hydroxypropylphosphonic acid epoxidase (HppE), which uses a mononuclear iron site to catalyze a unique epoxidation; and SyrB2, a mononuclear iron protein involved in natural product biosynthesis.