STRUCTURAL STUDIES OF WEST NILE ENVELOPE

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 西尼罗河病毒是美国一种新兴传染病的病原体，NIAID将其视为B类优先病原体。目前还没有针对西尼罗河病毒的许可疫苗或特异性抗病毒治疗药物;因此，迫切需要开发疫苗、化学预防剂或化学治疗剂来预防或治疗越来越多的确诊病例，药物治疗将成为国家生物防御武器的重要组成部分。我们已经结晶了西尼罗河病毒包膜蛋白，并收集了3。在100 oK的数据集，尽管低温条件差，在旋转阳极发电机。此后，我们改进了低温条件，目前正试图通过分子置换解决相问题。我们希望提高SSRL的分辨率，如果必要的话，通过MAD/SAD实验获得相位。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. West Nile virus is the etiological agent of an emerging infectious disease in the United States and is considered by the NIAID to be a category B priority pathogen. There is no licensed vaccine or specific antiviral therapeutic available for West Nile virus; therefore, the development of a vaccine, chemoprophylactic, or chemotherapeutic agent is urgently needed to prevent or treat a growing number of diagnosed cases and a drug treatment would be an important part of the nation's biodefense armament. We have crystallized the West Nile envelope protein and have collected a 3. dataset at 100oK, despite poor cryogenic conditions, on a rotating anode generator. We have since improved cryo conditions and are currently attempting to solve phases through molecular replacement. We hope to improve the resolution at SSRL, and if necessary, obtain phases through MAD/SAD experiments.