SAXS TOWARD STRUCTURE DETERMINATION OF ACETOACETATE DECAROXYLASE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 乙酰乙酸脱羧酶是第一个从机理上研究的酶，并用于生产丙酮的生物技术应用。获得的9.8埃分辨率的EM图显示了这种十二聚体酶非常不寻常的组装-一个具有D6对称性的中空球，直径100埃，由所有的β-折叠组成。拟议的SAXS实验将允许我们调整EM的对比度函数，并测量一个样品的精确形状和体积，以便重新调整我们的EM数据。远距离的目标是使用电磁相位对我们的高分辨率(1.8埃)X射线数据进行相位处理。由于分期过程要等到SAXS完成后才能进行，因此快速访问非常重要。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Acetoacetate Deacrboxylase is the first enzyme to be studied mechanistically, and to be used in a biotech application for producing acetone. An EM map obtained to 9.8 angstrom resolution shows a highly unusual assembly for this dodecameric enzyme- a hollow sphere with D6 symmetry, 100 angstroms in diameter made up of all beta sheet. The proposed SAXS experiment will allow us to modulate the contrast function for EM and to measure the precise shape and volume of one sample in order to re-scale our EM data. The long range goal is to use the EM phases to phase our high resolutuion (1.8 angstrom) X-ray data. As the phasing process is held up until SAXS can be done, rapid access is important.