LASER-CROSSLINKING OF MICRO-RNPS ON THEIR MRNA TARGET

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 MicroRNAs(MiRNAs)与属于ArgAerte家族(AGO)的蛋白质结合。MiRNAs通过与其mRNA靶标中发现的miRNA识别元件(MRE)进行碱基配对来发挥作用。我们以前在MRE上引入了位点特异性的光交联核苷酸，这样紫外线照射就可以在MRE和它的结合蛋白之间形成共价键。我们想要研究利用四倍频的Nd：YAG激光器来进行这种光交联。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. MicroRNAs (miRNAs) are bound to proteins that belong to the Argonaute family (Ago). miRNAs function by base pairing with miRNA-recognition elements (MREs) found in their mRNA targets. We previously incorporated site-specific photocrosslinkable nucleotides nto an MRE so that UV irradiation forms covalent bonds between the MRE and its binding protein. We would like to investigate the use of the fourth harmonic of a Nd:YAG laser for this photocrosslinking.