DEVELOPING A PUBLIC CRYOEM REPOSITORY DATABASE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在过去的十年里，用电子冷冻显微镜确定的结构的数量有了显着的增长；这一趋势预计还会加快。为了提供一个合适的接口来描述和归档低温EM结构，我们与PDB和EBI合作开发了一个低温EM本体，不仅描述了结构本身，而且描述了实验条件和相关的元数据项。我们希望就沉积3D密度图和来自CryoEM研究的原子模型所需的数据项建立社区共识。此外，我们还致力于改进目前的低温电子显微镜数据收集和沉积方法，以便实验者拥有一个通用的、易于使用的、一站式的低温电子显微镜结构沉积门户。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The past decade has seen a remarkable growth in the number of structures determined using electron cryomicroscopy; this trend is expected to accelerate. In order to provide a proper interface for describing and archiving cryoEM structures, we work with PDB and EBI in developing a cryoEM ontology to describe not only the structure itself, but also experimental conditions and pertinent meta-data items. We look to establishing a community consensus on the data items needed for deposition of 3D density maps and atomic models derived from cryoEM studies. Additionally, we are focused on improving the current cryoEM data collection and deposition methods so that the experimentalists will have a common, easy to use, one-stop portal for cryoEM structure deposition.