DEVELOPING A PUBLIC CRYOEM DATA REPOSITORY DATABASE

开发公共 CryOEM 数据存储库数据库

• 批准号: 8361073
• 负责人: Helen M Berman
• 金额: $ 2.45万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361073
• 关键词: Archives; Biological Models; Collection; Communities; Cryoelectron Microscopy; Data; Databases; Deposition; Educational workshop; Electron Microscopy; Funding; Goals; Grant; Image; Imagery; Maps; Metadata; Modeling; National Center for Research Resources; Principal Investigator; Proteins; Research; Research Infrastructure; Research Personnel; Resources; Source; System; United States National Institutes of Health; Validation; base; cost; density; macromolecule; reconstruction; research study; structural biology; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our goal is to create a robust system for the collection, validation, annotation, distribution and visualization of data derived from cryoEM experiments and analyses. We will provide researchers with an integrated "one-stop shop" for the deposition and assessment of metadata, maps, and models, as well as a comprehensive and quantitative description of the deposited data and its linkage to other relevant databases for end-users. We are building on and significantly enhancing the systems currently operated by the wwPDB for the deposition and distribution of 3D atomic models, and the systems at the EBI for the deposition and distribution of electron microscopy based reconstruction maps. The end result will be the full integration of cryoEM experimental data into the PDB archive as managed by the Worldwide Protein Data Bank (wwPDB, http://wwpdb.org). In addition, the investigators at the National Center for Macromolecular Imaging in Houston will also develop the necessary computational and visualization tools so that the cryoEM researchers can use them to validate the maps and models, and the archived density maps and models can be easily viewed by the public. Through this establishment, we continuously seek the advice from the various experts in the cryoEM and structural biology community through workshops.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们的目标是创建一个强大的系统，用于收集，验证，注释，分发和可视化来自cryoEM实验和分析的数据。我们将为研究人员提供一个综合的“一站式商店”，用于存放和评估元数据、地图和模型，并为最终用户提供对所存放数据及其与其他相关数据库的链接的全面和定量描述。我们正在建立并显著增强目前由wwPDB运行的用于沉积和分发3D原子模型的系统，以及EBI用于沉积和分发基于电子显微镜的重建图的系统。最终结果将是将cryoEM实验数据完全整合到由全球蛋白质数据库（wwPDB，http://wwpdb.org）管理的PDB档案中。此外，休斯顿国家高分子成像中心的研究人员还将开发必要的计算和可视化工具，以便cryoEM研究人员可以使用它们来验证地图和模型，并且公众可以轻松查看存档的密度图和模型。通过这个机构，我们通过研讨会不断寻求cryoEM和结构生物学领域专家的建议。