FURTHER DEVELOPMENT OF DIRECT DIGITAL IMAGING SYSTEMS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 开发用于中压电子显微镜(IVEM)的数字成像系统是本核心项目的目标。之前，我们讨论了为JEM-3200F安装新的可伸缩1K x 1K快速相机的问题。这款快速扫描相机安装在JEM-3200FX上，提供了出色的图像。我们今年报告了Tietz视频和图像处理系统(TVIPS)在资源JEM-4000EX上新安装的1k x 1k，F114-R快速扫描CCD。这款相机具有12帧/秒的全分辨率(25帧/秒，2 x 2)读数，12位模数转换器，在奈奎斯特提供超过12%的对比度。该系统最有用的特征之一是能够提供近乎实时的快速傅立叶变换，为矫正散光提供无与伦比的反馈。随着这种广角实施的性能得到改进，IVEM已经做好了充分的准备，可以提供大格式、高质量的视频反馈，以支持远程显微镜和自动断层扫描(如FasTomo等)。在过去的一年里，我们已经将快速扫描相机集成到NCMIR的通用远程显微镜控制软件套件中。随着围绕JEM-3200仪器的大部分软件集成和开发完成，我们预计新的F114-R在JEM-4000EX系统上的集成将会很快。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Developing digital imaging systems for intermediate voltage electron microscope (IVEM) is the objective of this core project. Previously, we discussed the installation of a new retractable 1K x 1K fast camera for the JEM-3200F. This Fast-scan camera was installed on the JEM-3200FX and is providing excellent images. We report this year on a new installation of the 1k x 1k, F114-R fast scan CCD from Tietz Video and Image Processing Systems (TVIPS) on the resource JEM-4000EX. This camera features a 12 frames-per-second readout at full resolution (25 frames-per-second, binned 2 x 2), 12 bit analog-to-digital converter, delivering greater that 12% contrast at Nyquist. One of the most useful features of this system is the ability to provide nearly real time fast Fourier transforms, providing unmatched feedback for correcting astigmatism. With the improved performance of this wide-angle implementation, the IVEM is well equipped to deliver large format, high quality video feedback in support of telemicroscopy and automated tomography (e.g. FasTomo, etc.). In the past year, we have integrated the fast-scan camera into NCMIRs Generalized Telemicroscopy suite of control software. With much of the software integration and development completed around the JEM-3200 instrument, we anticipate the integration of the new F114-R on the JEM-4000EX system to be quick.