ARCHITECTURE AND REGULATION OF THE BUDDING YEAST KINETOCHORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 有丝分裂纺锤体的功能关键依赖于着丝粒，这是一种连接纺锤体微管和着丝粒DNA的蛋白质结构。虽然在不同的生物体中已经发现了大量的动粒蛋白，但这些蛋白质是如何组织起来连接染色体和纺锤体微管的，以及是否有任何结构和组织主题对于远亲生物的动粒是共同的，这些都是关键的悬而未决的问题。在这里，我们使用亲和层析和质谱学来生成动粒蛋白相互作用的图谱。绘制了动粒蛋白的磷酸化位点图，并分析了体内突变这些位点的功能后果。这将使人们对发芽酵母动粒的结构和调控有更深的了解。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Mitotic spindle function critically depends on the kinetochore, a proteinacous structure that serves to connect spindle microtubules to centromeric DNA. While numerous kinetochore proteins have been identified in diverse organisms, how these proteins are organized to connect chromosomes to spindle microtubules, and whether any structural and organizational themes are common to kinetochores of distantly related organisms, are key unanswered questions. Here, we use affinity chromatography and mass spectrometry to generate a map of kinetochore protein interactions. Phosphorylation sites of kinetochore proteins are mapped and the functional consequences of mutating these sites are analyzed in vivo. This will allow a deeper understanding of the architecture and regulation of the budding yeast kinetochore.