PROTEOMIC ANALYSIS OF THE HUMAN KINETOCHORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们目前正在使用亲和标记策略来研究人类动粒的组成和组织，以分离已知和新的人类动粒蛋白和动粒蛋白复合体。我们开发了一种联合方法来检查蛋白质的细胞定位，并从人类组织培养细胞中进行高亲和力纯化该蛋白质(定位和亲和纯化-LAP标签)。使用这种方法，我们已经分离了20多个人动粒蛋白，包括至少10个新的动粒蛋白。这项正在进行的分析将试图描述人类动粒蛋白的全部范围，并检查它们的相互作用和组织。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are currently investigating human kinetochore composition and organization using an affinity tagging strategy to isolate known and novel human kinetocore proteins and kinetochore protein complexes. We have developed a combined approach to examine the cellular localization of a protein and conduct a high affinity purification of that protein from human tissue culture cells (Localization and Affinity Purification - LAP tag). Using this approach, we have isolated more than 20 human kinetochore proteins, including at least 10 novel kinetochore proteins. This ongoing analysis will attempt to characterize the full range of human kinetochore proteins and examine their interactions and organization.