New Phosphated Polymers Prevent Microbial Colonization

描述(申请人提供)：本研究项目的目标是开发新一代义齿基托聚合物，可以控制微生物定植和预防义齿引起的口腔炎。这些新聚合物将通过在聚甲基丙烯酸甲酯[PMMA]中加入磷酸基团来合成，以防止义齿感染。新聚合物中的磷酸基团将为唾液阳离子抗菌剂选择性地吸附到义齿表面提供所需的阴离子表面。这将导致义齿表面形成一层防护性的膜层。目前可用的PMMA由于缺乏离子表面和防御性义齿薄膜成分，不能抑制微生物的定植。本研究项目涉及：1)合成含有不同数量的磷酸盐基团和非磷化PMMA的磷化PMMA并表征聚合物；2)使用组蛋白5和防御素片段评估该聚合物吸附和解吸唾液中抗菌剂的效果；3)使用白色念珠菌、血链球菌、口腔链球菌、戈登链球菌和变形链球菌测定吸附了抗菌药物的磷酸盐聚合物的杀菌活性；4)描述聚合物的物理和机械性能，这些聚合物对唾液中的抗菌剂表现出高亲和力，并根据美国食品和药物管理局/国际标准化组织的指南抑制微生物在聚合物表面的黏附；5)评估聚合物标本对人口腔上皮细胞系、人牙龈成纤维细胞系和标准L-929细胞系的体外细胞毒性，以确定牙科材料的安全性要求。相关性：这项拟议的研究将确定新的、安全和负担得起的磷酸化PMMA聚合物，这些聚合物可以作为义齿基托或义齿基托衬里材料用于预防和治疗义齿感染。这些新型聚合物将表现出与正常口腔表面相当的物理、机械和生物性能，并将对现有PMMA聚合物治疗假牙感染所涉及的费用产生重大影响。这些新聚合物的使用可以改善大量假体使用者的口腔健康。磷酸化的PMMA聚合物在临床和商业应用方面具有很大的潜力。

DESCRIPTION (provided by applicant): The goal of this research project is to develop a new generation of denture-base polymers that could control microbial colonization and prevent denture-induced stomatitis. These new polymers will be synthesized by the incorporation of phosphate groups within poly (methyl methacrylate) [PMMA] to prevent denture infection. Phosphate groups in the new polymers will provide the required anionic surface for the salivary cationic antimicrobials to be selectively adsorbed onto the denture surface. This will result in the formation of a defense pellicle layer on the denture surface. The currently available PMMA, due to the absence of ionic surface and the defense denture pellicle component, does not inhibit microbial colonization This research project involves: 1) Synthesis of phosphated PMMAs containing varied amounts of phosphate groups and non-phosphated PMMA and characterization of polymers; 2) Assessment of the efficacy of this polymer to adsorb and desorb salivary antimicrobials using histatin 5 and a defensin fragment; 3)Determination of the microbicidal activity of antimicrobials adsorbed phosphated polymers using Candida albicans, Streptococcus Sanguis, Streptococcus oralis, Streptococcus gordonii and Streptococcus mutans; 4) Delineation of the physical and mechanical properties of polymers that exhibit high affinity for salivary antimicrobials, and inhibit microbial adhesion to the polymer surface following the ADA/ISO guidelines; 5) Assessment of the in vitro cytotoxicity of polymer specimens against a human oral epithelial cell line, human primary gingival fibroblast cells, and the standard L-929 cell line to ascertain the safety requirements for Dental materials. Relevance: This proposed research will identify new, safe and affordable phosphated PMMA polymers that could serve as denture-base or denture base lining materials for the prevention and treatment of denture infection. These new polymers will exhibit physical, mechanical and biological properties comparable to those of normal oral surfaces, and will have significant impact on the expenses involved in the treatment of denture-induced infection associated with the existing PMMA polymer. The use of these new polymers could improve the oral health of a significant population of prosthesis users. The phosphated PMMA polymers could have great potential for clinical and commercial applications.