Inhibition of Candida albicans and Mixed Salivary Bacterial Biofilms on Antimicrobial Loaded Phosphated Poly(methyl methacrylate).
Inhibition of Candida albicans and Mixed Salivary Bacterial Biofilms on Antimicrobial Loaded Phosphated Poly(methyl methacrylate).
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DOI：
10.3390/antibiotics10040427
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发表时间：
2021-04-13
期刊：
影响因子：
--
通讯作者：
Tahriri M
中科院分区：
文献类型：
--
作者：
Dentino AR;Lee D;Dentino K;Guentsch A;Tahriri M
生物膜在牙列相关性义齿性口炎的发生中起着至关重要的作用。在这项工作中，微生物粘附到聚（甲基丙烯酸甲酯）（PMMA）和含磷酸盐的PMMA的抑制已被检查。C.在存在或不存在抗微生物剂（氯化十六烷基吡啶[CPC]、KSL-W、组胺素5 [His 5]）的情况下，在裸露和唾液预处理的PMMA表面上比较白色念珠菌和混合唾液微生物生物膜。使用四种C.评估白色念珠菌口腔分离物以及混合唾液细菌和24小时生物膜的代谢活性，并使用活/死染色和共聚焦显微镜进行确认。随着磷酸盐密度的增加，生物膜代谢减少（15%：p = 0.004; 25%：p = 0.001）。在存在或不存在唾液膜的情况下，CPC在15%磷酸化圆盘上的负载显示生物膜代谢的大幅降低（p = 0.001）。不带电的PMMA上的唾液膜仅增强CPC的抗菌活性。CPC还在不同条件下对混合唾液细菌生物膜表现出显著的抗微生物活性，当与人工蛋白膜（生物素半强度）组合时，显示CPC（350 μg/mL）的有效功效。
Biofilms play a crucial role in the development of Candida-associated denture stomatitis. Inhibition of microbial adhesion to poly(methyl methacrylate) (PMMA) and phosphate containing PMMA has been examined in this work. C. albicans and mixed salivary microbial biofilms were compared on naked and salivary pre-conditioned PMMA surfaces in the presence or absence of antimicrobials (Cetylpyridinium chloride [CPC], KSL-W, Histatin 5 [His 5]). Polymers with varying amounts of phosphate (0–25%) were tested using four C. albicans oral isolates as well as mixed salivary bacteria and 24 h biofilms were assessed for metabolic activity and confirmed using Live/Dead staining and confocal microscopy. Biofilm metabolism was reduced as phosphate density increased (15%: p = 0.004; 25%: p = 0.001). Loading of CPC on 15% phosphated disks showed a substantial decrease (p = 0.001) in biofilm metabolism in the presence or absence of a salivary pellicle. Salivary pellicle on uncharged PMMA enhanced the antimicrobial activity of CPC only. CPC also demonstrated remarkable antimicrobial activity on mixed salivary bacterial biofilms under different conditions displaying the potent efficacy of CPC (350 µg/mL) when combined with an artificial protein pellicle (Biotene half strength).
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DOI：
10.1196/annals.1384.030
发表时间：
2007-01-01
期刊：
ORAL-BASED DIAGNOSTICS
影响因子：
--
作者：
Oppenheim, Frank G.;Salih, Erdjan;Helmerhorst, Eva J.
通讯作者：
Helmerhorst, Eva J.
DOI：
10.1002/jbm.820291015
发表时间：
1995-10-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
EDGERTON, M;RAJ, PA;LEVINE, MJ
通讯作者：
LEVINE, MJ
影响因子：
3.7
作者：
Li, L.;Finnegan, M. B.;Shi, W.
通讯作者：
Shi, W.
影响因子：
3.1
作者：
EDGERTON, M;SCANNAPIECO, FA;LEVINE, MJ
通讯作者：
LEVINE, MJ
DOI：
10.1034/j.1600-0528.2000.028002141.x
发表时间：
2000-04-01
影响因子：
2.3
作者：
Budtz-Jorgensen, E;Mojon, P;Deslauriers, N
通讯作者：
Deslauriers, N