CELL SORTER/FLOW CYTOMETRY CORE FACILITY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 细胞分选器核心将提供骨髓或皮肤干细胞分离和细胞悬液中供体细胞分析的专业知识。凯文·约翰逊是干细胞/分选机的全职技术人员；此外，马克·杜纳将他的一定比例的时间投入到这一核心中。这一核心采用了来自Cytomation的MoFIO高速细胞分选器。这种分选机能够进行干细胞分离、单细胞沉积和大事件分析。这个核心被安置在一个专用的房间里。FACS设施还配备了FACS扫描仪和FACS Calibur(BD仪器)。这两台机器都可以作为项目所需的任何分析工作的备份。研究人员将与核心合作，并准备好他们的细胞群体，以便进行分类或分析。核心将常规分离Lin-Rho(Lo)Ho(Lo)、Lin-Hoechst侧细胞和Lin-SCA+CD45-细胞，并分析GFP+或CFDA-SE阳性细胞群体。该核心将为科布雷所有开展分离和分析工作的项目提供服务。核心仍然致力于开发新的干细胞和加强已知的干细胞分离。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Cell Sorter Core will provide expertise in marrow or skin stem cell separations and analysis of donor cells in cell suspensions. Kevin Johnson is the full time stem cell/sorter technician; in addition, Mark Dooner devotes a percentage of his time to this core. This core features a MoFIo highspeed cell sorter from Cytomation. This sorter is capable of carrying out stem cell separations, single cell deposition and large event analysis. This core is housed in a dedicated room. The FACS facility is also equipped with a FACS scan and a FACS calibur (BD instruments). Both machines can act as a backup for any analysis work required by the projects. Investigators will work with the core and prepare their cell populations for sorting or analysis. The core will routinely separate Lin- Rho(lo)Ho(lo), Lin - Hoechst side populations and Lin - Sca+ CD45- cells and analyze populations for GFP+ or CFDA-SE positive cells. The core will serve all projects in this COBRE carrying out both separations and analysis work. The core also remains dedicated to the development of new and the enhancement of already known stem cell separations.